Study On Hydrogen Production From Fruit Biomass By Photosynthetic Bacteria

The fruit production of China ranked first in the world, as representatives, the production of apple and watermelon reached 20 and 70 billion kg respectively,and because of the bad weather, the long-distance transportation, not easy to save and other reasons, a lot of fruit went bad eventually. It was found that rotting fruit was eaten would poison or even kill human and animal, so rotting fruit could only be thrown away. Hydrogen production from rotting fruit by photosynthetic bacteria could not only eliminate the waste of resources, but also reduce environmental pollution and produce clean and renewable sources, which had begun to attract people’s attention.This paper studied the effects of different p H value on the growth of photosynthetic bacteria HAU-M1, made the standard curve of dry biomass on OD660 nm, and established the regression equation of photosynthetic bacteria with Logistic model.This paper investigated the effect of material to liquid ratio, p H regulation, initial p H, light intensity, fermentation temperature, inoculum amount on hydrogen production by photosynthetic bacteria from apple puree, watermelon and corn stalk, established the best process parameters of hydrogen production by photosynthetic bacteria, compared and analyzed hydrogen production from rotting fruit and stalk by photosynthetic bacteria.The results showed that:1) The p H value had significant effects on growth and hydrogen production of photosynthetic bacteria, with the increase of p H value, the photosynthetic bacteria biomass increased gradually. When the p H value is 8, the photosynthetic bacteria biomass reached the maximum, 543.714 mg/L, the best inoculation time is 84-96 h.2) Hydrogen production by photosynthetic bacteria increased with the increase of apple puree concentration, Acid-base regulation would decrease hydrogen production from apple puree by photosynthetic bacteria. The best process parameters of hydrogen production from apple puree by photosynthetic bacteria were: apple puree and distilled water quality ratio, 1:4, the initial p H value, 7, the light intensity, 3000 lx, the temperature, 30℃, the inoculation, 40%. The results of this study provided scientific reference to further improve hydrogen production by photosynthetic bacteria from fruits and vegetables biomass theoretically and technically.3) Cellulase had good effect on enzymatic hydrolysis of watermelon peel, reducing sugar concentration of watermelon juice increased significantly after treated by cellulose. Acid-base regulation would decrease hydrogen production by photosynthetic bacteria because acid-base regulation may weaken photosynthetic bacteria activity or even directly killed them.4) Using BBD model, Optimize the hydrogen production from enzymatic saccharification of corn stalk by photosynthetic bacteria. The best process parameters of hydrogen production from enzymatic saccharification of corn stalk by photosynthetic bacteria were: p H, 5.43, temperature, 30.8 ℃, the amount of cellulose, 70 mg/g. The maximum hydrogen yield of BBD model was 149.39 m L, the maximum hydrogen yield was 29.88 m L/g.