The Roles Of TaATG8in The Process Of HR Induced By Leaf Rust Infection With Wheat

Hypersensitive reaction (HR) is one of the important forms of plant resistance topathogen, that is cell rapidly death near the pathogen infect parts and pathogen, formingnecrotic lesion, which make the growth of pathogenic bacteria can not get the rightenvironment and nutrition and death, in this case it has been confined to a certain range tobe extended. At the same time, HR must pass a special control in a certain range ofmechanism finely, so that the host damage control to a minimum. But so far this regulatorymechanism is not clear. Autophagy, refers to cells under the condition of nutrientdeficiency or stress, through the degradation of cell senescence long-lived protein,damaged cell structures and organelles, for the reconstruction of the cells, regeneration andrepair provide the necessary raw materials, make cell recycle and reuse come true, is a kindof important biological defense and protection mechanisms. Cell autophagy functions inplants are constantly being revealed, especially in the role of immune response, hasattracted widespread attention. About the role of autophagy in HR there are two oppositepoints of view: cell autophagy protects cells from cell death and cell autophagy promotescell death. The present data show that the two hypotheses are likely to be correct,depending on cell type, the kinds of stimulation and cell state of development. Our grouplong been engaged in Wheat Leaf Rust infection mechanism, in the Process of Triticumaestivum resistance to Puccina recondita infection will also produce large amounts of HR,HR Task Force induced mechanism has been around a lot of work, but the mechanism ofHR limit poorly understood. This study of Triticum aestivum Puccina recondita-interaction system as the research object, use of Triticum aestivum (Triticum aestivum l.)varieties loughlin10and Puccina recondita (Puccinia recondita f.sp. tritici) physiologicalincompatibility combination consisting of260,165, and affinity, investigate autophagy inthe role of Triticum aestivum resistance to Puccina recondita infection induced HR.1. The atg8iconic form gene of autophagy was cloned by the method of RT-PCRfrom the wheat seedlings’ leaves.2. Constructed prokaryotic expression vector, Get a lot atg8fusion protein, byimmunization New Zealand rabbits, Obtain High titer rabbit antiserum, and prove that theserum and ATG8fusion protein was specific binding. 3. Using RT-qPCR and Western Blotting technique detected the expression changes ofTaATG8gene in wheat after leaf rust infection by different time points. The results showedthat: The gene expression at the RNA level before the24h did not differ between twocombinations, But in36h incompatible combinations of the gene expression wassignificantly higher than the affinity combination, and this difference was more apparent at48h, after the gene expression at the transcriptional level tends to fall, until120h minimum;Expression of protein in incompatible combinations after inoculation, gradually increasesuntil the highest at36h, then after the decline, but the expression seems to have bounced at96h, The affinity combination has been no significant change. Preliminary indicating thatthe gene may be involved in the defense responses process during the Triticum aestivumresistance to Puccina recondita infection.4. In order to further study the function of autophagy, using virus induced genesilencing (VIGS) silence the key genes TaATG8autophagy formed, Puccina reconditainduced HR extension, autophagy may have negative regulation of HR. This research helpsus better understand the role of autophagy in the plant immune response, to providescientific theoretical basis for disease resistance breeding and the prevention and controlstrategies.