¹³C Stable Isotope Tracer-based Spectral Analysis Techniques And Metabolism Of Sugar Metabolism Of Pear （Pyrus Bretschneideri Rehd. Cv. Dangshansuli） Fruits

On the classification between Pyrus ussuriensis and Pyrus bretschneideri,dangshansuli is one of the largest pear varieties for cultivation area, which has a longcultivation history in Dangshan city Anhui and China. Dangshansuli is a kind of fruit tree,which belongs to rosaceae and its’ sorbitol is different form photosynthetic product. In themeanwhile, it is particular to use horticultural crops fruit as the research object and theconnotation of the fruit quality characteristics and its formation mechanism is differentfrom other crops. Researching the key metabolic pathways related with sorbitolmetabolism can provide an appropriate research system research to develop metabolicresearch on main quality characteristics of the composition.The fruit of DangShansuli was collected as trial material When130days after blossom falling, then they were disposed by¹³C-glucose injection method, and cultivation of frtwafer combining with¹³C-glucose feeding processing method, and then the samples aftertreatment were gathered in different time, at last metabolite profiling analysis was used to analyze sugar and important fruit characteristics constituents witch closely related to sugarmetabolism of bud mutation fruits and unmanifest fruits.The significant differences in different pears was found through analysis the sample by GC-MS and based on the NIST and Wiley database to analytic the structure of the metabolic components in the GC-MS fingerprinting.We got metabolism of quantitative data through dealing with GC-MS spectrumdenoiseing, deconvolution, baseline calibration and peak matching, summarized data offruit¹³C-glucose injection experiment application of principal component analysis （PCA）and partial least squares （PLS） analysis for data processing, then we obtained mutant fruitat fruit ripe period to generate large amounts of sugar, fruit stands in stark contrast with thecontrol group. Organic acid demand has increased in the process of fruit ripening, butfound in the variation and the control group the fruit they present for precancerous changeslittle, gap in fruit acids generation between variation and control group t is small. Middlevolatility, variable fruit increased ratio of organic acids is greater than the control fruits;late total acids were little changed, for the variation of fruit acids levels drop ratio isgreater than the control group fruits. Through3D map shows the experimental wafer andinjection experiments with high-sugar mutant and natural fruits can achieve good patternclassification, and access to the injection experiment samples for test material contributionto the larger value metabolite information （VIP）, the top20larger variations were compared in analysis of these metabolites.Based on several glucose comparison of standard mass spectra, concluded that thecharacteristic peaks of glucose metabolism related markers of¹³C glucose wereparticipated in the metabolism of mutation fruit to detect marked metabolites formed in themetabolism of sugar in the fruit. Detected downstream of glucose metabolism with taginformation of several metabolites, mutation fruit was found a significant difference withthe control group. In the control group during the first hour appeared more marked than thevariation fruit species and production of metabolites. With prolonged incubation time, thecontrol group labeled metabolites detected and types and contents decreased, but variationgroup after showing a little more than the first phenomenon. Description the control groupfruits easier join of exogenous glucose consumption and quickly produce rich metabolites,glucose metabolic mutation rate is relatively slow, but produces a wider range of sugars,non-mutant and mutation fruits’ carbon metabolic flux analysis and the differencesbetween them.