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Fusairum And Fungicides Of Malus Hupehensis On Physiological And Biochemical Characteirstics

Posted on:2014-12-12Degree:MasterType:Thesis
Country:ChinaCandidate:G L ZhaoFull Text:PDF
GTID:2253330425977173Subject:Pesticides
Abstract/Summary:PDF Full Text Request
Currently is the country entered a period of aging in most Apple advantage producingarea, facing the old Orchard varieties to replace and update problem in orchard. Cultivatedland area and economic and regional issues such as branding restrictions limiting replantingobstacle as a challenge of fruit industry development. Biological factors is one of theimportant causes of sickness. Malus hupehensis is one of Apple’s common stock and fruit treecultivation physiology of the materials used in the study, based on their seedlings to testmaterial in this article, using hydroponic methods to combine and potted plants, determinationof Fusarium oxysporum on biochemical and physiological indices of Malus hupehensisgrowth processes of the impact study on inhibitory effect of chemical fungicides on Fusarium,analysis on mitigation effects of continuous cropping obstacles. To further reveal thepathogenic mechanism of Fusarium and based on new information provided and thus providea basis to solve Apple’s problems.1. The use of hydroponic experiments, research mainly in the continuous cropping soilseparation of Fusarium oxysporum Fusarium fungus, Fusarium moniliforme strains, layers,and putrid skin culture filtrate of Fusarium on growth inhibition of Malus hupehensisseedlings. Study results indicate that filtrate treatment can significantly inhibit plant growth,plant height, root length, root-tip and root surface area decreased significantly. Filtrates ofFusarium can promote seedling growth slightly while concentrations of10%, culture filtratesstarts to inhibit the growth of seedlings when concentrations of25%, with increasingconcentration and processing time, enhanced the inhibition. Protective enzyme activity inearly to rise, as the culture filtrate concentration increase and extend the processing time,superoxide dismutase (SOD) activity and malondialdehyde (MDA) content has beenincreased peroxidase (POD) and catalase (CAT) activity by different ranges of declines.Membrane lipid peroxidation levels increase conductivity increased membrane permeabilityincreases.2. M. hupehensis seedlings growing in Nutrient solutions were treated with Toxins ofFusarium oxysporum filtrate at different concentrations. Effect of the toxin filtrate ofFusarium on plant morphological and physiological characteristics was investigated. Theresults showed that the toxin filtrate of Fusarium improved seedlings growth at10%andinhibited the growth of seedlings at25%. Inhibition rate of the toxin filtrate of Fusarium onseedlings enhanced with its concentration and processing time increased. The early treatmentof protective enzyme activity increased. With the increasing of the concentrations of toxin filtrate and the extension of processing time, SOD activity and MDA content increased,Activities of POD and CAT appeared different declines. Since membrane lipid peroxidation,conductivity and cytolemma leakage increased.3. Continuous cropping obstacle is in the process of apple update one of the difficultproblems in, continuous cropping obstacle cause complex, explore reduce apple effectivemeasures of continuous cropping obstacles with theoretical value and practical significance.Access bacteria kernel of sterilization soil medicament treatment respectively mancozeb WP2.5a.i g/10kg soil; Carbendazim WP2.5a.i g/10kg soil; Fludioxonil WP1.25a.i g/10kgsoil in Malus hupehensis seedlings growth, root vigor, chlorophyll content and protectiveenzyme activity influence. The results show that after the fungicide treatment pingyi sweet teagrowth promoting effect significantly, root vigor, chlorophyll content, protective enzymeactivity had different degrees of improvement.. Fungicide can inhibit the sickle in the soilfungi on plant pathogenic role of promoting plant growth, reduce the harm.4. By pot experiment, study on inoculation and control of continuous cropping soil, soiland differences between the respective control. Experimental results show that. In inoculationon soil fungicides shown role in promoting more significantly than in the continuous croppingsoil, specificity of inhibition is stronger.
Keywords/Search Tags:Fusarium, Toxin, Malus hupehensis Rehd., Replant problem, Fungicides, Protective enzyme
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