Genetic Diversity Of Emerald Ash Borer Agrilus Planipennis Fairmaire In China

The emerald ashborer，Agrilus planipennis Fairmaire (Coleoptera: Buprestidae: Agrilus)is an important wood-boring beetle attacking ash trees (Oleceae: Fraxinus spp.).In the1960s’， EAB severely infested F.americana and F. pennsylvanica in the northeast regions ofchina.In recent years，the tendency of the EAB’s harm has exacerbated. The EAB used in thisstudy were collected in2011～2012as larvae and adults from ash trees. Specimens werecollected from native populations in Changchun, Liaoning, Shenyang, Tianjin and Beijing ofChina. EAB total genomic DNA was extracted from larval tissue. Two partial sequences (totalapprox.1000pb) of the COI genes were amplified from EAB individuals using two primers.By analysis of mtDAN COI variation and refer to the literature we can get the geneticdiversity and geographical speread of Asian populations and North American. The resultswere as follows:1The genetic diversity and geographical spread of EAB populations in the five locations ofChina.Analysis of mtDNA COI variation DNA sequencing of a1000bp fragment of the COIgene was carried out for83EAB individuals from the5populations of China, and thenanalyzed the sequence variability of the COI gene and genetic differentiation among them. Asingle common mitochondrial (Hap1) was found. Six other haplotypes (Hap2～Hap7) wereobserved in other populations. Tieling has the most of haplotypes (Hap1, Hap5～Hap7).Shenyang has three haplotypes (Hap1～Hap3). Population’s haplotype were performed todetermine the most likely geographical speread. The identity of COI pairwise sequences was99%among the genetic populations of EAB, which indicated a low genetic diversity. EABgenerally spread from north to south, such as it speread from Tieling to Tianjin.2The genetic diversity and geographical speread of EAB populations in Asian and NorthAmerican.For all the EAB populations in Asian and North American,9distinct geographicpopulations were defind based on geographic proximity(country or provence). These9populations that were used for the analyses are: North American, Beijing, Hebei, Jilin,Liaoning, South Korea, Heilongjiang, Tianjin, and Japan. Japan population’s haplotype wasdifferented from the common haplotype by21base pair changes. Analysis of the EABmtDNA sequence data yielded two groups in the resulting network. The haplotype (Hap9)from the single individual from Japan differed from the common haplotype and separated into its own group. The remaining haplotypes formed the second group. Other EAB specimensfrom North America、China and South Korea had the common haplotype. So the abovegeographic populations can defind into four parts: North American population、South Koreapopulation、China population and Japan population. These EAB were mutual communicationexpet Japan population.