Studies On The Anther Culture And Microspore Culture Technology Of (Brassiea Campestris L. Ssp Chinensis L)

In8different genotype extraction varieties as experimental materials of anther and microspore culture. Sampling time of flowering Chinese cabbage anther culture anther culture of the material genotypes, additional material type and concentration, medium composition and culture methods and materials genotype, hormone types and concentration ratio of microspore culture and agar concentration the regenerated seedlings embryoid system. Apex tablet at the same time and measurement The leaf stomatal guard cell size of the pollen regenerated plants Ploidy identification, comparison of cytology and agronomic traits Haploid and diploid plants, and regeneration groups ploidy structure analysis, the results show that:1. Flowering Chinese cabbage microspore developmental stages are closely related to the appearance of its flower bud length2.5～3.5mm, petal anther1/2to4/5, most of the spores in the single nuclear sidelined period, in this case, the anthers microspore culture period.2. Anther culture medium supplemented with0.5%activated carbon can greatly improve embryoid induction rate test two varieties embryo rate.3. Medium supplemented with sucrose concentration of10%, the best on the anther culture embryoid the induced effect.4. The glutamine concentration in the medium within a certain range is not very sensitive, the optimum concentration of600to800mg/L.5. Anther culture, replacing the previous solid culture method of solid-liquid two-layer culture, can greatly increase the frequency of embryoid inductions6. The small spore concentration level of microspore culture of a great impact, the effect of concentration of2×105个/ml in the top7. Using orthogonal test the embryoid induction rate ratio microspore culture hormone type and concentration of1. Omg/L6-BA+0.4mg/L NAA is flowering Chinese cabbage microspore culture optimal embryoid induction medium hormone combination.8. Inducing embryoid regenerated seedlings, in order to improve the regeneration the seedling rate requires an appropriate increase in the content of the agar medium,10g/L is appropriate, seedling rate can reach60to70%。9. Haploid plants of flowering Chinese cabbage chromosome number10, the diploid number of chromosomes is20; small guard cells of the length and width of the same parts of the pollen leaf lower epidermis guard cells than female plants, and between guard cell length and the width of the existence of significant differences. The haploid and diploid plants exist differences in agronomic traits. The higher rate of natural doubling of the regenerated plants flowering Chinese cabbage,55to65percent, a lower percentage of haploid plants accounted for30to40%, without artificial doubling production.