Study On Semen Cryopreservation Of Black Silky Chicken

Semen freezing is the foundation of livestock and poultry artificial insemination and species gene pool preservation, But the current poultry semen cryopreservation, still without a reasonable, effective freezing procedures, can make poultry frozen semen to stabilize the fertilization rate, This is why poultry, frozen semen main reason for not industrialization. So the research of poultry semen and to further research and developmenStraw semen simple portable refrigeration device and method,In view of the existing straw frozen semen technology depends on the complex and expensive production equipment, Fumigation of liquid nitrogen and variety of simple method, Especially for the same batch of samples cannot simultaneously realize different freezing rate freeze issues. Research and development of a device for frozen semen or cell and freezing method, device is simple in structure, can realize the standardization, mass production, especially it can realize a plurality of samples with different freezing rate freeze, improve the efficiency of research and small batch production frozen semen. At present, the device has obtained a patent for utility model, the method of the invention patent application has entered the stage of substantive examination.Study of black Silkies frozen sperm,dilutions,equilibrium time and freezing rate. Using BPSE, Lake’s and FEB respectively as semen dilution group, in which BPSE and Lake’s group after freezing sperm vitality no significant difference, but there is significant difference with FEB group (P<0.01). Using BPSE as the diluent adding cryoprotectants, balance of1min,10min,20min,30min and60min, frozen thawed sperm motility into a downward trend. No differences in the balance of lmin and10min clock sperm motility,using BPSE as the diluent, adding cryoprotectant balance in10under the conditions of min, after thawing,1cm,2cm, comparison between3cm and4cm group,1cm group and sperm motility were higher than those in other groups (P<0.01). The black silky chicken semen freezing, to a final concentration of6%DMA as cryoprotectant, dilution of BPSE and Lake’s, balancing selection in10min, in the patent device conditions,1cm7min from liquid nitrogen frozen, thaw in37.8 ℃water bath, can obtain good experimental effect.Study of black silky chicken semen freezing damage detection method.The experimental find fresh semen, before freezing semen and frozen thawed semen, sperm DNA damage rate increased significantly, respectively is6.2%,6.4%and19.8%. The comet tail length and width ratio assumes the trend of escalation, and frozen semen (30.1%) before and after thawing semen (30.9%) had no obvious differences. The proportion of damaged sperm membrane, fresh semen (2.9%) and before freezing semen (5.6%) no obvious difference, but after freezing semen film breaking rate was significantly higher than that of the former two. Fresh semen, semen and frozen semen frozen before the activity is respectively52.1%,51.9%and19.8%. The experiments show that cell viability, membrane integrity and the DNA intergrity is index, the results are consistent, in addition to illustrate the damage occurred mainly in the freezing thawing process, but not before freezing process and cryoprotectants on seminal fluid toxicity.