Screening And Identification Of Seed Quality Trait Mutants In Rice(Oryza Sativa, L.)

Rice has now become one of the world’s most important food crops. As a model crop, it also provides the basis for monocots researching. Rice gene function studies play vital roles in the areas of both scientific research and life. Building rice mutants is a direct and effective way for researching rice genes. Meanwhile, using physical radiation and chemical mutagenesis can improve mutation frequency which is much higher than the natural mutation, and also can conduct a lot of purposeful selections. These establish the foundation of building materials in the laboratory.Through physical-ray mutagenesis and chemical mutagenesis MNU treatment, we get a lot of mutagenesis M1generation seeds of N22,93-11and Dianjingyou1that are three conventional rice species.Through screening mutant traits in the field, as well as examining seed traits after harvesting. The result is mutant materials25copies about agronomic traits. It builds a batch of rice mutant materials for the research of functional genes and provides favorable materials in the research of functional genes and rice genetics.After harvesting mutagenic M2seeds and peeled observation that is the main observation of rice endosperm mutants’ traits, we choose the chalky, opaque materials and other related traits. The result is mutant materials752copies about the endosperm. N22,283copies;9311,196copies; Dianjingyou1,293copies.After seed selection, we determine the protein content change of being selected starch mutants, namely conduct the SDS-PAGE electrophoresis. We screen glutelin and prolamin mutants and focus on rice protein synthesis pathways. After numerous generations of validation, we eventually eliminate the effects of environmental factors and find genetically stable protein mutant. Through a large number of SDS-PAGE electrophoresis analysis, we finally select homozygous mutant rice protein gpa4-1.The mutation phenotype are etiolating in the tip of leaves, fluory endosperm, and glutelin precursor increase. After hybridization with the N22, an F2population was constructed for positioning cloning. This gene was initially located in a17.5cM interval on the third chromosome. Genes predicted in the17.5cM interval, have not been reported before, suggesting gpa4-1is a novel glutelin precursor accumulation mutant. The laboratory may find new materials.