Phenotypes And Mechanism Investigation Of Arabidopsis Expressing GBVE1Gene Against Verticillium Dahliae

Cotton Verticillium wilt, caused by Verticillium dahliae, is a soil-borne vascular high risk disease. The disease causes the cotton leaf yellow, even death of whole seedling. The desease was first reported by Carpente in1914in Virginia Upland cotton. More than a century, the prevention and treatment of the disease have never stopped, and achieved some preogresses. Prevention methods of cotton Verticillium wilt include chemical pesticides, reasonable cultivation methods, biological control and the cultivation of resistant varieties. Cultivation of resistant varieties is considered to be the most economical and environmentally friendly and effective approach, however, disease resistant breeeding is usually difficult because the resistant cultivars sources are limited. Previously, we have cloned a gene, Gbvel, from the island cotton using molecular cloning methods, which was a homologous of tomtato resistant Vel gene and could significantly enhanced the resistant levels of V. dahilae in transgenic Arabidopsis. However, its mechanisms are still largely unknown.To investigate Gbvel-transgenic Arabidopsis resistant mechanisms, we firstly screened the positve transgenic Arabidopsis lines and analyzed their resistant levels to V. dahilae by inoculating with fungal conidia. The results showed that the trasgenic lines were more resistant, especially for the isolate V991. We found that the callose deposition, cell death and reactive oxygen species accumulation in the process of V. dahliae infection were signifantly enhaced in the transgenic plants. We then compared the gene expressional profiles between the trasngenic lines and wilt type. We identified1204genes that were upregulated in the trasngenic lines, while543genes were down-regulated. Our results provide a fundmental basis of how Gbvel gene mediates Verticillium resistance in Arabidopsis.