Inlfuence Of Probiotics On The Growth, Water Quality And Nitrogen And Phosphorus Budgets In Young Sea Cucumber (Apostichopus Japonicus) Culture

In this article, the sea cucumber (Apostichopus japonicus) was selected, and the effects of four kinds of probiotics on growth performance, water quality, nitrogen and phosphorus budgets of sea cucumber in room culture were studied. In addition, we discussed the effects of water exchange rate on the sea cucumber.1. A54-day feeding trial was performed to investigate growth enhancing effects and the water quality of four probiotics in sea cucumber (Apostichopus japonicus). The sea cucumber body weight0.7760±0.0046g. EM and LP mainly component is Lactobacillus. BP mainly component is Bacillus. HA mainly component is Yellow humic acid. Every probiotics set three concentrations. Every concentration set four replicates. There were two control groups, one group without probiotics, the other group without sea cucumber and probiotics. Every control group set six replicates. The results show that:it can be seen through the water quality, adding5.0ml/m3EM on degradation nitrite (NO2--N) works best; adding2.0g/m3LP on degradation ammonia (NH4+-N) works best. By comparing the survival rate can be seen, adding10.0ml/m3HA show the highest survival rate; addition of2.0g/m3LP works best on improving specific growth rate of sea cucumber. To sum up, LP is the most suitable probiotics in the indoor rearing young cucumber, the concentration of2.0g/m3works best.2. A36-day feeding trial was performed to investigate quality and growth enhancing effects of water exchange rate in sea cucumber (Apostichopus japonicus) at the use of probiotics. The sea cucumber body weight1.2310±0.0046g. The (LP) mainly component is Lactobacillus. A total of six experimental groups were3d,5d,8d,12d,15d, and do not change the water groups set up, every group set three replicates. The results show that:it can be seen through the water quality, at the water exchange rate of3d and5d, work best on degradation nitrite (NO2--N) and ammonia (NH4+-N). By comparing the survival rate can be seen, water exchange rate had no significant impact on the survival of sea cucumber; specific growth rate, the water exchange rate of3d (1.5645±0.0688) works best, but it had no significant impact with5d.The results demonstrated that the water exchange rate of5d was the best choice on the cucumber culture.3. In this study, we investigate nitrogen and phosphorus budgets enhancing effects of four probiotics in sea cucumber (Apostichopus japonicus) culture.On the nitrogen input, feed (56.6%)> cucumber (25.6%)> seawater (17.7%), the difference between treatment groups was not significant (P>0.05). On the phosphorus input feed (88.2%)> cucumber (10.0%)> sea water (1.8%), the difference between treatment groups was not significant (P>0.05); On the nitrogen output, bait fecal output accounted for30.5%-45.1%nitrogen, water output accounted for35.0%-43.0%nitrogen, sea cucumber output accounted for23.0%-42.6%nitrogen, phosphorus output, bait fecal phosphorus output accounted for most part, accounted for73.6%-82.4%, followed by cucumbers,12.3%-21.4%, and finally the sea, accounting for4.6%-6.2%. The LP, concentration of2.0g/m3nitrogen conversion efficiency is highest; the EM group5.0ml/m3phosphorus efficiency is highest.4. In the case of using lactobacillus probiotic LP, different change water rates contribution of nitrogen and phosphorus input, feed accounts for66.3%to74.5%and95.7%to96.6%respectively, the water accounts for0.6%to6.7%and0-0.3%respectively, sea cucumber accounted for24.9%to28.5%and3.3%-4%respectively. In terms of nitrogen and phosphorus output, remnant waste output is the main contribution of nitrogen, phosphorus, followed by the sea cucumber, the last is the sea water. Including3d change water rate of nitrogen and phosphorus into cucumber efficiency is the highest.