| Eggplant (Solanum melongena L.)originated in the tropical regions of southern Asia, for its first domesticated in ancient India, the Chinese long history of cultivation of eggplant, eggplant second origin, with rich eggplant germplasm. Eggplant fits warm. In greenhouse or early spring field culrure it often is back of pollination because of low temperature, low illumination or no pollination agency.This situation results in loosing flowers and fruits,reducing benefit. The varieties of parthenocarpic eggplant can overcome the poor pollination and other adverse effects of shattering and form the fruit in the end. Therefore, it is very important to screen and locate the parthenocarpic gene of eggplant for breeding parthenocarpic eggplant. To find the molecular markers linked with parthenocarpy, it will be easy to identify the parthenocarpy eggplant in the seedling stage and shorten the breeding process, which has an important role for eggplant parthenocarpy assisted breeding.In this study, SRAP was used to alysis the parthenocarpic gene linkagean of Solanum melongena. The parthenocarpic line (D-6), the non-parthenocarpic line (896) and the F2generation group were as materials in the experiment, which were used to construct the extreme gene pools, that was parthenocarpic pool and non-parthenocarpic pool. The main results were as follows:1. The modified CTAB method was used to extract the whole genome DNA of Solanum melongena. The results showed that the genomic DNA fragment was better, the DNA concentration was relatively higher, and the DNA quality was within the standardr ange of values, thus the genomic DNA was fully applicable to analysis of SRAP.2. Used genomic DNA of Solanum melongena as template, several affecting factors such as Mg2+, dNTPs, primer, template DNA and Taq enzyme were combined to choose the best concentration of SRAP-PCR reaction system. The optimized SRAP-PCR reaction system (10μL) of Solanum melongena, was as follows: concentration of Mg2+was2.0mmol/L, concentration of primer was0.5mol/L, concentration of Taq DNA polymerase was0.75U, concentration of dNTPs was0.20mmol/L, concentration of DNA template was50ng/μL.10pairs of SRAP primers were selected to confirm and the results showed that the system coule be used to amplify the whole genomeof Solanum melongena in SRAP.3.525pairs of primer combination were obtained through combining21forward primers and25reverse primers, of which24pairs displayed parental polymorphic primers after amplification banding analysis. Moreover, six pairs of primer combinations appeared stable differences in the two extremes pools of parthenocarpy and non-parthenocarpic gene by banding analysis. Six parthenocarpy plant and six non-parthenocarpic plant of the two extremes pools were amplified by using the six pairs of primer combinations to do the SRAP-PCR, and only the prime me13em17was related to parthenocarpic phenotype of Solanum melongena.4. By using the me13em17primer, a specific band of about327bp was amplified in all non-parthenocarpic lines, and a specific band of about275bp was amplified in all parthenocarpic lines. The mark appeared on the non-parthenocarpic lines was named as327me13em17, and the other one was named275me13em17. The linkage distance with the parthenocarpy sites between these two markers was calculated through JoinMap4.0, and the results were15.89cM and11.43cM.5.200single plants of F2populations were tested using the me13em17primer. The results showed that among200single plants, about143were non-parthenocarpic lines, and57were parthenocarpic lines. The separation ratio between non-parthenocarpic lines and parthenocarpic lines in F2generation groups was close to3:1.Through x2test we though D-6’s parthenocarpy is controlled by single recessive nucleus gene. |
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