Volatiles Identified From Leaves Of Different Tomato Species&Metabolitics Analysis Among Tomato Core Germplasm

Tomato is a world-wide genus with approximately18species (http://tgrc.ucdavis.edu/). Most tomato species possess leaves compounds and emit special odors. The flavor of tomato fruit has been studied intensively; however, less attention has been paid to the leaves. Striking differences regarding odor were found during our investigation on the germplasm resources of tomato species.Leaf volatile components of different wild and cultivated tomato accessions were analyzed using Head Space-Solid Phase Micro Extraction (HS-SPME) combined with Gas Chromatography-Mass Spectrometer (GC-MS).It was found that the orange odor emitted by accessions LA1316and LA1318of Solanum chmielewskii was mainly associated with ocimene and y-terpinene, while the unpleasant smell of accessions LA1353and LA1777of S. habrochaites probably came from elemene, which is an effective anticancer compound.Further, the transcriptional level of terpene synthase gene family was investigated by using quantitative reverse transcription-PCR. According to the gene expression analysis, we have found candidate genes having a positive relation to the major components.Those results promote us to speculate the mechanism of terpenes biosynthesis and provide useful cues for further study and application on terpenes.