A Study On Germplasm Resource Of Salvia Miltiorrhiza F. Alba:Investigation, Collection And Comparison

Salvia miltiorrhiza Bge.f.alba, mainly distributed in Zhangqiu and Laiwu of Shandong province, is a forma of S. miltiorrhiza, belonging to the family of Labiatae. Its root is a Chinese herbal medicine, having the functions of promoting blood circulation, improving menstruation, relieving pain. It is beneficial to coronary heart disease, angina pectoris, irregular menstruation and insomnia, especially specific to thromboangiitis obliterans. In order to protect this rare germplasm, its wild resource was investigated and some comparative studies were conducted in this thesis. The main results are as follows:The forma was found sporadically distributed in Laiwu, Zhangqiu, Taian and Linyi, etc, mainly grown in the shadow of hilly land with thick soil.The wild resource was transplanted in experimental plot, and Five types were detected when the wild plants were transplanted in our plant gardens, e.i. large oval leaf type, large acute leaf type, small oval leaf type, small acute leaf type, and sterile type. These five types were planted separately, and their biological characteristics were observed, including leaf shape, leaf color, flowering characters, and so on. At the seam time, a series of agronomy traits were investigated, such as the weight of fresh and dried root, and the contents of tanshinone Ⅱ A and salvianolic acid. In addition, aphid-resistance and propogation were also investigated.Large acute leaf type gained the highest root yield, with fresh weight of268g per plant (dry weight96g), that is significantly superior to other variants. However, the difference in drying rate was not significant among different types. Large oval leaf, large acute leaf and sterile types were showed some resistance to aphid.The content of tanshinone ⅡA was0.75%in large acute leaf type,0.62%in small oval leaf type, all significantly higher than that of the others. The highest content of salvianolic acid B11.9%was found in large acute leaf type, and the lowest in the sterile type, however, there was no obvious difference among the other types. In addition, the content of tanshinol was almost identical.The rooting percentage of stem cutting of large oval leaf, small oval leaf and large acute leaf types was all over85%, and their seed germination was also relatively high. Tissue culture experiments showed that the medium MS+2. Omg/L6-BA+1.0 mg/LNAA(or0.5mg/L) could be used as induction medium, MS+KT1.5mg/L+NAA0.2mg/L as subculture medium, and1/2MS+IBAO.2mg/L suitable for plantlet rooting. After acclimation, the survive rate of the plantlets is over95%.In summary, a good strain of S. miltiorrhiza f. alba, large acute leaf type was selected, and its propagation was improved, which lay a good foundation for further development and utilization of this excellent germplasm resource.