| Rapid propagation technique system of Salvia miltiorrhiza Bunge was established by the study of its tissue culture from 1999 to 2002, and field experiment proved that the quality of the plantlets was superior and quantity were improved. The results were as follows:1. The highest survival of 0.2-0.3cm shoots from Salvia miltiorrhiza Bunge was observed onl / 2MS medium with 6-BA0.4mg/L.2. The best medium for regeneration of leaves from test tube plants and greenhouse plants was 1 / 2MS medium with 6-BA 3mg/L .the leaves from greenhouse plants regenerated later compared with those from test tube plants, but the inductive rates of adventitious shoots from both kind of leaves were not significantly different.3. The best sub-culture medium for Salvia miltiorrhiza Bunge was 1 / 2MS medium with 6-BA0.4mg/L.Multiplication rate differed from season to season: the explants cultured from February to April resulted in a multiplication rate of 6.03, whereas those cultured resulted in a multiplication rate of 3.57 from September to November.4. Using 2 / 3MS medium containing O.lmg/L 6-BA ,stronger plantlets could be produced.5. In in vitro rooting trial, the rooting rate of plantlets was 98% on 2 / 3MS4- IBA0.2 + NAA0.2 after 10 days compared with 68% after 20 days. In ex vitro rooting trial, survival rate of plantlets was 75% by dipping their stem base in solution containing 50mg/IBA and NAA compared with 52~55% by dipping their stem base in solution containing 75mg/IBA and NAA.6. The field trial proved that the seedlings from tissue culture were superior to the ordinary seedlings by cutting. The content of Tanshinone IIA in crops from tissue culture seedlings reached 2.54%, 20.39% higher than that of contrast.
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