QTL Mapping For Seed Related Traits In Soybean(Glycine Max L.Merr.)

Soybean （Glycine max L.Merr.） is one of the most important oil crops. Yield ofsoybean in China only can meet one third of domestic consumption.Therefore highyield breeding become the superordinate breeding goal. Seed weight is one ofimportant factors affecting soybean yield. With the development of agriculturalmodernization, mechanization gradually improve. Seed shape affect efficiency ofmachinery utilization by affecting sowing regularity. Seed shape was measured byseed length, seed width and seed thickness.Those traits were quantitative traitcontrolled by multiple genes, by studying the related QTL loci, realize the yieldcharacters of molecular marker assisted breeding, soybean industry in China hasimportant application significance.It has play an important application significance by study seed weight and seedshape in improving soybean yield and efficiency of machinery utilization. It wasreported that seed weight and seed shape were quantitative traits. In this study themolecular marker information of216F6RILs from direct and reciprocal crossesbetween Jidou12and Heidou（ZDD03651） were used to construct the genetic linkagemaps using163SSR polymorphic primers. QTL locus of stable control seed weightand seed shape in multiple environments was identified, combined with phenotypicdata in multiple years and multiple locations. In order to analyze QTL additive andepistatic effects in seed weight and seed shape, various methods were used.Excavating excellent allelic variation of the QTL locus provide basis for widely usingQTL locus in multiple environment. Development molecular markers chainingexcellent allelic variation play material foundation for molecular assisted breedingselection.The results as follows:1.Soybean genetic linkage group using163SSR primers in20linkage groupwere constructed, containing1516cM soybean genome. The average distancebetween markers was9.3cM.2. A total of5QTL locus related to100-seed weight were detected, respectively qSDW-A2-1、qSDW-A2-2、qSDW-C2-1、qSDW-D1b-1and qSDW-D2-1. qSDW-C2-1was detected in Shijiazhuang and Sanya, the highest contribution rate was11.85%.qSDW-D2-1was detected in Shijiazhuang in multiple years, the highest contributionrate was13.01%. In addition,3pairs QTL locus for seed weight epistatic effect weredetected, respectively.The epistatic contribution rate were among7.80%and16.76%.3. A total of29QTL locus related to seed shape were detected. qSL-D2-1、qSW-C2-1、qSW-D2-1、qST-A2-2、qST-C2-1，qST-D2-1，qSLW-C1-1、qSLW-D1b-3、qSLT-A2-2、qSLT-F-1�'�qSWT-D1b-1were detected in multiple environments, thecontribution rate among7.71%and14.76%. QTL synergistic genes of seed length,seed width and seed thickness were from Jidou12. QTL synergistic genes of seedlength/width, seed lenght/thick and seed width/thick were from Heidou.4.140allelic variation were detected in5seed weight QTL locus.11excellentallelic variation were sreened from140allelic variation. SSR markers ofsat₄06-212、sat₄06-214、sat₄06-216、satt119-124、satt281-227、sat₂79-259、B-2-304-245、 satt301-199、 satt301-259were linkage to these excellent allelicvariation.