The Effect Of Huangkui Capsules On Renal Tubulointerstitial Fibrosis In Mice With Unilateral Ureteral Obstruction

Objective：To observe renal interstitial pathological changes and the expressionof transforming growth factor beta1（TGF-β₁） and α-smooth muscle actin（α-SMA） inrenal tissue of unilateral ureteral obstruction（UUO）mice by therapeutic interventionwith Huangkui capsules,and to investigate the effect of Huangkui capsules on renaltubulointerstitial fibrosis.Methods：Km male mice54, the average body weight is18-22g. The mice wererandomly divided into sham operation group （n=18）,UUO group （n=18） andHuangkui capsules treatment group （n=18）.They were scarificed on3,7,14days afterestablishing UUO model.Obstructed kidney were examined by HE staining,Massonstaining, immunohistochemisty staining and reverse tanascription-polymerise chainreaction（RT-PCR）. Renal interstitial pathological changes and the expression ofTGF-β₁and α-SMA in renal tissue were observedResults：（1） Our results showed that the renal interstitial damage and renalinterstitial fibrosis area were significantly increased in UUO group and treatmentgroup compared with the sham operation group （P<0.05）, and reduced in treatmentgroup compared with UUO group（P<0.05）.（2） Expression of the TGF-β₁and α-SMAwere signify-cantly increased in UUO group and treatment group compared with thesham operation group （P<0.05）, and increased in UUO group compared withtreatment group （P<0.05）.（3） The expressive levels of TGF-β₁mRNA and α-SMAmRNA were significantly increased in UUO group compared with the othertwo groups （P<0.05）. The expressive levels were significantly increased in treatmentgroup compared with the sham operation group （P<0.05）.（4） Correlate analyzeshowed that TGF-β₁and α-SMA were positively correlated with renal interstitialpathological changes（P<0.05）.Conclusion：TGF-β₁,α-SMA and renal tubulointerstitial fibrosis are closelyrelated; Huangkui capsules can significantly reduce the expression of TGF-β₁andα-SMA,thus it could suppress fibrosis of renal interstitial.