The Study Of Molecular Genetic Basis Of Weak D Phenotype Individuals In Fujian Province

Objective To investigate the distribution and molecular basis of weak Dphenotype individuals in fujian province,the southern-eastern area of china.Method All samples were tested by serologic blood grouping assays for theirphenotypes. Weak D phenotype individuals were identified from the non-relatedblood donors and patients by indirect antiglobulin test(IAT).The genotype of RHDwas studied by using polymerase chain reaction with sequence-specific primer(PCR-SSP),and all10RHD exons were sequenced. RHD zygosity was detectedthrough PCR-SSP.Result1.Thirty-two samples were identified as weak D phenotype by blood groupserologic tests,with a frequency of0.0114%in fujian province.2.Weak D type20、Weak D RHD(R113R)、Weak D type15、Weak D RHD(N340I)、Weak D RHD(L320L)、Weak D RHD(F214L)、Weak D RHD(K409K)、Dva、DⅥtypeⅢ were found in2、3，2，3，1，1，1，1，2weak D phenotypeindividuals,respectively.The molecular mechanism was undiscovered inthe rest of16individuals.3. RhC，Rhc，RhE，Rhe antigens detected by serologic test were Ccee in18samples（56.25%），CcEe in4（12.5%），CCEe in4（12.5%），ccEe in3(9.375%)，others in3(9.375%),which was consistent with the results genotyped by PCR-SSP.4. RHD+/RHD+homozygotes were detected in20samples,and the others wereRHD+/RHD-heteozygotes.Conclusion There were significant differences in molecular mechanism of weak Dphenotype individuals among fujian indigenous population compared to those of otherareas in china. Distribution characteristics of Weak D phenotype werediversified,which had9kinds of phenotypes, including weak D type20, Weak D RHD(R113R)，weak D type15, Weak D RHD(N340I)，weak D RHD (L320L), weakD RHD (N320I), weak D RHD (K409K), Dva, DⅥtype Ⅲ, each phenotype beingnot dominant, the distribution being more dispersed.So, it is necessary to strengthenthe study of the molecular mechanisms of the weak D phenotype, and clear its anti-Dimmune occurrence and different molecular genetics background of weak Dphenotype individuals, which would be helpful to make proper transfusion strategies,to prevent to be immuned by inject ing anti-D immunoglobulin into women duringand after pregnancy.This was particularly important clinical significance for safe,scientific, rational clinical blood tranfusion and the prevention, diagnosis, treatmentfor neonatal hemolytic disease.