The Effect Of Probiotics On The Insulin Resistance In The Rats With Nonalcoholic Fatty Liver Disease

Objective:To investigate the effect of probiotics on the insulin resistance in the ratswith Nonalcoholic fatty liver disease. Serum lipid、liver homogenate lipid、glucose、serum transaminase、insulin and plasma endotoxin were measured andthe pathological changes of liver was observed to expound the influence ofintestinal flora on insulin resistance of non alcoholic fatty liver in rats. And toinvestigate probiotics’s prevention and treatment effectiveness that in thedevelopment of insulin resistance of non alcoholic fatty liver and the mechanismsof action.Methods：Thirty male SD rats were randomly divided into normal control group,NAFLD model group, probiotics treated group(treated with bifico210mg to eachrat everyday through the way of gastric lavage) after adaptive feeding1week. Atthe end of fourteen week, all rats were executed. And cheeck on the weight.Separate the serum and the liver tissues to measure the level of serum TC、TG、FBG、AST、ALT and liver homogenate TG、TC、GLU with full automaticbiochemical analyzer. Measure the level of FINS with the ELISA kit andcalculate IRI and ISI to evaluate the degree of insulin resistance with FBG andFINS. Measure the level of plasma endotoxin with limulus lysate test. The degreeof liver steatosis was assessed under light microscope after HE staening.Results:1. Compared with control group, the liver index in model group wassignificantly increased(P <0.05). Administration of probiotics couldn’t obviouslyreduce the liver index(P>0.05). Results of the differences of body weight gainweren’t statistically significant between the three groups(P>0.05).2. Compared with control group, the serum lipid and liver homogenate lipidwere significantly increased in model group existing disorders of lipid metabolism (P <0.05). Administration of probiotics could significantly improvedisorders of lipid metabolism (P <0.05).3. Compared with control group, the level of transaminase in model groupwas significantly increased(P<0.05). Administration of probiotics couldsignificantly reduce the level of transaminase(P <0.05).4. Compared with control group, the level of FBG、FINS、IRI weresignificantly increased(P <0.05) and ISI was significantly reduced(P <0.05) inmodel group existing insulin resistance. Administration of probiotics couldsignificantly improve insulin resistance(P <0.05).5. Compared with control group, the level of plasma endotoxin wassignificantly increased in model group existing endotoxemia(P<0.05).Administration of probiotics could improve endotoxemia(P <0.05).6. Compared with control group, there was different degree of hepaticsteatosis in model group, mostly in severe level(P <0.05)under light microscopeafter HE staening. Administration of probiotics could significantly attenuate thehepatic steatosis, mostly in moderate level(P <0.05).Conclusions：1. Non-alcoholic fatty liver model in rats was sucessfully prepared by fedwith high-fat diet.2. The level of plasma endotoxin was higher in NAFLD model rats than innormal rats, suggestting that endotoxin might be more increasely absorbed intothe blood, and then form endotoxemia triggering inflammation, leading insulinresistanced and finally involving in the development of NAFLD.3. Probiotics could ease endotoxemia、improve insulin rensistance anddecrese steatosis gradings might through regulating the balance of intestinal florato decrese preduce and absorption of endotoxin.