Study On Biomechanics And In Vitro Culture Experiment For Tissue Engineering Scaffold Of The Defect Cranium

The restoration of the defect Cranium occupies an important place in bone tissueengineering and the tissue engineering scaffold for the defect cranium is now still instudy. The biomechanical and biological properties of the scaffold are the key issuefor clinical application. This paper is devoted to a deep study on the two aspects basedon the previous work of our group. The main contents are as follows.First of all, different porosity cranium scaffolds are first established to supportbiomechanics performance in finite element analysis and experimental study. Andthen rapid prototyping and gel casting molding are used to produce three-dimensionalbiological scaffold of the defect cranium.Secondly, in order to improve the mechanical properties of the scaffolds,Bioglass is introduced combining with optimal gel casting molding processparameters by orthogonal test. The shrinkage performance influenced by Bioglassadding and the adding order in the molding process is analyzed.Finally, the biological properties of the fabricated scaffold are detected byscanning electron microscope, X-ray diffraction analysis, energy spectrum analysisand in vitro culture experiment, which is to verify the rational biocompatibility of thecranium scaffold by the proposed design and fabrication method.Test results show that the36%porosity bionic scaffold has good mechanicalproperties, the mechanical strength and material component of the-TCP/BGscaffold is beneficial to the cultivation of the cell culture in vitro. It lays a theoreticalfoundation for the application and development of the cranium tissue engineeringscaffolds.