Studies On Quality Control And Effective Fraction Purified Of Liguistrum Lucidum

Fructus Ligustri lucidi (Chinese name Nvzhenzi) is the plant of Oleaceae andcome from the dried fruits of Ligustrum lucidum Ait.，which is a kind of traditionalChinese medicine. It has the effect of nourishing liver and invigorating the kindney,promoting the eyesight and blackening the hair.Use for dizziness and tinnitus,sorenessand weakness of waist and knee,premature whitening of hair. In recent years, thestudy of Ligustrum lucidum Ait. has made some progress, but needs to be improved inthe method of quality control and the purification of effective part.Ligustrum lucidum Ait.was studied above two questions in the article, the first,Six compounds were isolated and identified as oleanolic acid, lupeol, acetyloleanolicacid, beta-sitosterol, Ligustroflavone, salidroside. Salidroside and Ligustroflavone arethe essential component of Ligustrum lucidum Ait., The second, The HPLC method ofLigustrum lucidum Ait. is built as the salidroside and Ligustroflavone.The linearregression equation of salidroside is A1=24393X+73.75and0.9998, And the linearrelationship in the range of0.0625mg/mL-1.00mg/mL. The linear regressionequation of Ligustroflavone is A2=17468X-328.2and0.9990, And the linearrelationship in the range of0.125mg/mL-2.00mg/mL. Average recoveries ofSalidroside and Ligustroflavone were97.70%and97.30%, And RSD were1.03%and0.66%. The third, Optimized extraction and purification method of Ligustrum lucidumAit. were built as salidroside and Ligustroflavone, The optimal extraction method is10times the amount of60%ethanol was refluxed three times,every time for45minutes. The optimal purification method is use AB-8macroporous resin, theconcentration of the sample is0.6g/mL, sample flow rate for1mL/min and diameterto height ratio is1:9, water cleaning, and then60%ethanol whose elution volume is5BV can be eluted completely. The effective parts of Ligustrum lucidum Ait. isacquired, And the content of salidroside and Ligustroflavone in the Effective parts ofLigustrum lucidum Ait. are5.4%and21.3%detected by HPLC.