The Anti-cancer Study Of Flavonoids Of Scutellaria Baicalensis Georgi On Cervical Cancer In Vitro

Cervical cancer is a common gynecological malignancy, which seriously threatenslives and health of women. Chinese medicine is winning more and more approvalbecause of unique anti-tumor advantages. Scutellaria baicalensis Georgi is a traditionalChinese medicine in China, and flavonoids are effective active ingredients. Flavonoidshave good anti-tumor effects, but anti-tumor activity on cervical cancer has been studiedin vitro incompletely.In this research, effects and mechanisms of anti-cervical cancer of Scutellariabaicalensis flavonoids were studied fully and deeply in vitro. Flavonoids were extractedfrom Scutellaria baicalensis by ethanol refluxing, separated and purificated with methodof alkali solution and acid isolation, AB-8macropore absorbed resin. Logarithmic phaseHeLa cells cultured in vitro were treated with high, medium and low dose flavonoids atconcentration of250,175and100μg/ml. Cell viability was detected by Trypan bluestaining and MTT assay. Proliferation and migration were examined with cell woundhealing assay, at the same time, the rate of apoptotic cells and DNA content wereanalyzed by morphological observation and flow cytometry. Expressions of Cyclin B1and CDK1were detected by Western blot, and Caspase-3activity were detected withcolorimetric method.The results showed extraction rate of purified flavonoids was3.68%. With baicalinas standard, the content of flavonoids was21.18%. With the treatment of flavonoids,proliferation and migration ability of HeLa cells was inhibited significantly, at the sametime, the percentage of cells in S phase increased and the expression of Cyclin B1andCDK1protein decreased, finally Caspase-3was also activated in time and dose dependentmanner. Flavonoids might down-regulate the expression of Cyclin B1and CDK1proteinat G2/M regulation point of cell cycle, thus, HeLa cells were arrested in S phase to inhibitproliferation of HeLa cell. Moreover, it could induce apoptosis of HeLa cells byactivating the proteolytic enzyme Caspase-3activity. The mechanisms of anti-cervicalcancer of Scutellaria baicalensis flavonoids in vitro were revealed preliminarily, which provided a strong basis for development of natural anti-tumor medicine.