Influence Of Elemene On Expressions Of Pc-Met In Ascites Hepatoma Cell Line H22

Objective:To investigate the antitumor mechanism of elemene in asciteshepatoma cell line H22.Methods:After injecting ascites hepatoma cell line into their right oxter,thirty-two mice were divided into four groups: Normal Saline negative group(NS),high-dose elemene group, low-dose Elemene group and5-Fu positive group. Afterthirty days, tumor was obtained and the inhibitory rates of solid tumor were observed.The expression of pc-Met was examined by immunohistochemistry straining method invivo and immunofluorescence technique in vitro.Results:The inhibiting rate were41.6%and22.5%in high-dose elemene groupand low-dose elemene group, which were obviously higher than NS control group (P <0.05). After H22cells were treated with various concentrations of drug, the expressionof pc-Met were increased in three drug groups compared with NS group. In the tumortissues, the high-dose element group and low-dose element both have more positivepc-Met cells than NS group. Rank-sum test showed that expression of pc-Met wasincreased in drug groups, the difference between drug groups and NS group hadstatistical significance but the comparison between element group and5-Fu group (P>0.05) was not statistically significant. The expression of pc-Met was significantlyincreased in a dose and reaction time dependent manner in both vivo and vitro.Conclusion: The mechanism of elemene significantly inhibiting effect on asciteshepatoma cell line H22cells is possible relative to the expression level of pc-Met.