Detection Of Tissue Factor And Microparticle-associated Tissue Factor And Clinical Significance In Acute Myeloid Leukemia

Objective:This study was to detect the expression of tissue factor (TF) and microparticle-associated tissue factor (MP-TF) in the patients with acute myeloid leukemia (AML)in order to analyze clinical significance in the haemostatic disorder..Methods:The peripheral blood in64case of acute myeloid leukemia patients with newly diagnosed was collected into citrated vacutainer tubes.There are22cases of APL patients,42cases of AML patients,22cases of AML with DIC and42cases of AML without DIC.13cases of AML patients with bone marrow complete remission.15cases of APL patients with bone marrow complete remission including12cases of the APL with DIC.34cases of normal controls were healthy blood donors.(1) The level of MP-TF in the patients with AML was detected by FCM using antibody TF-PE and Annexin-V-FITC.(2)The level of TF in the patients with AML was detected by enzyme-linked immunosorbent assay (ELISA).Results:(1) The level of MP-TF in the AML:The results of MP-TF in AML patients was significantly higher than that in the healthy adults(P<0.001). There were no significant difference between the level of MP-TF in the AML and acute promyelocytic leukemia(APL)(P>0.05). The level of MP-TF in the AML before chemotherapy was not different from that after chemotherapy. The results of MP-TF in APL patients bfore chemotherapy were higher than that after chemotherapy(P<0.05).including that in the APL with DIC. (2) The results of MP-TF in the patients with different coagulation indicators: The results of MP-TF in AML and APL with disseminated intravascular coagulation(DIC) were also no significant difference than that in AML and APL without DIC (P>0.05).The results of MP-TF in the patients with prothrombin time (PT) extents were higher than that in the patients with normal PT value(P<0.05). The level of MP-TF in the patients with abnormal fibrinogen degradation products (FDP)value was no statistically significant difference than that in the patients with normal FDP value (P>0.05)(3) The level of TF in the AML:The level of TF in the patients with AML was higher than that in the healthy adults(P<0.05). There were no significant difference between the level of TF in the AML and APL (P>0.05). The levels of TF in the AML and APL with DIC before chemotherapy were both higher than that after chemotherapy(P<0.001, P<0.05).(4) The results of TF in the patients with different coagulation indicators: The results of TF in the APL with DIC were no significant difference than that in the APL without DIC (P>0.05). The results of TF in the patients with abnormal FDP value were higher than that in the patients with normal FDP value (P<0.05). The level of TF in the patients with PT extents was no statistically significant difference than that in the patients with normal PT value (P>0.05).Conclusions:(1) The level of MP-TF in the patients with AML was high expression.The level of MP-TF in the patients with APL and APL with DIC decreased after chemotherapy. MP-TF could be used as a factor to monitor disease remission.(2)The results of MP-TF in the patients with haemostatic dysfunction such as abnormal PT value were increased significantly. MP-TF increases along with prolonged PT.The level of FDP in the patients with abnormal FDP value was also increased.MP-TF was associated with haemostatic dysfunction in the AML.