Effects Of Celecoxib And Sodium Butyrate On Colorectal Adenoma And Carcinoma Cell

Objective：we investigated the adenoma cells and colon cancer HCT-116cells inan attempt to know whether NSAIDs and sodium butyrate could be used as effectiveadjuvant for reducing cell proliferation, and to explore the possible mechanisms thereinvolved.Method: Adenoma cells and colon cancer HCT-116cells were treated withdifferent doses of celecoxib(100,200,400umol/L) sodium butyrate(0.5,1.0,2.0mmol/L)and celecoxib plus sodium butyrate(celecoxib100umol/L+sodium butyrate0.5mmol/L),we cultured for24,48and72h respectively. Cell prolifereation was determined by MTTassay, cell cycle and apoptosis were assayed by flew cytometey. The telomerase activitywas analyzed by PCR-ELSA. The expression of hTERT and COX-2mRNA weredetermined by RT-PCR.Result:1. compared with the control group, the prolifereation rates of adenoma cells andcolon cancer HCT-116cells treated by different doses of celecoxib and sodiumbutyrate were inhibited significantly. The inhibitory effect of celecoxib and sodiumbutyrate on cell prolifereation were in a time-and doses-dependent manner. Theinhibition effect of combination celecoxib with sodium butyrate enhanced obviouslythan that adds them alone (P <0.05).2. celecoxib and sodium butyrate exhibited doses-dependent induced the increaseof G1phase cells. Combined treatment group have statistically significant thansingle-agent group (P <0.05).3. After adenoma cells and colon cancer HCT-116cells were intervention bycelecoxib and sodium butyrate alone for48h, the apoptosis rate of cells were increased,with drug concentration increase, the effect get more obviously. The difference wasstatistically significant (P <0.05). Combined treatment group have statistically significant than single-agent group (P <0.05).4. There were found celecoxib and sodium butyrate could suppress the telomeraseactivity of adenoma cells and colon cancer HCT-116cells, and the effects wasdose-dependent. After celecoxib and sodium butyrate administration, the expression ofCOX-2and hTERT mRNA in adenoma cells and colon cancer HCT-116cells weredecreased, but their combination down-regulated it more significantly than signal(P <0.05).Conclusion:1. The growth of adenoma cells and colon cancer HCT-116cells were inhibited bycelecoxib and sodium butyrate, and the effects were dose-and time-dependent. Theanti-carcinoma effect was significantly enhanced by Celecoxib combined with sodiumbutyrate, and maybe its mechanisms were arresting cell cycle。2. The mechanisms of celecoxib and sodium butyrate inhibit cell growth should beinhibit, the expression of COX-2and hTERT mRNA transcription level, suppress thetelomerase activity, which block the cell cycle and apoptosis.