Clinical Significance Of Osteopontin And Matrix Metalloproteinase-9in Plasma Of Patients With Head And Neck Squamous Cell Carcinoma

Head and Neck cancer accounts for16.4%to39.5%of all tumors, and the happening rate of which is rising. Head and neck squamous cell carcinoma (HNSCC) corrects with smoking and human papillomavirus (HPV) infection, which affects the prognosis of patients. The early symptoms are not obvious to diagnose due to the anatomy, so it is hard to discover at early stage. Lymphatic metastasis and distant metastases can also occur through invasive growth locally and spread along the submucosal infiltration. Now radical surgery and preoperative or postoperative adjuvant radiotherapy are the main therapeutic methods for HNSCC, but curative effect evaluation is deficiency for objective, sensitive and specific detection index. The final diagnosis still require pathologic examination of biopsy. Therefore, how to increase the screening of HNSCC is the premise key to enhance the effect of treating HNSCC. Recently, osteopontin (OPN) and matrix metalloproteinase-9(MMP-9) has been improved to associate with tumor’s happening and development and play an important role in the invasion, metastasis of tumor. For example, some researchers indicate that high expression of OPN and MMP-9can be found in many tissues of malignant solid tumors such as stomach, large intestine and cervix. However, there are few reports on relations between OPN and MMP-9in plasma and diagnosis and treatment of patients with tumor,especially HNSCC. But the detection of OPN and MMP-9in plasma is simpler, quicker and harmless. This research discloses clinical significance in HNSCC patients’treatment, diagnosis, prognosis, evaluation and monitoring of OPN and MMP-9by detection of them in plasma of patients with HNSCC cancer.Objective:To investigate the clinical significance of osteopontin and matrix metalloproteinase-9(MMP-9) in plasma of patients with HNSCC.Methods:Plasma level of osteopontin and matrix metalloproteinase-9were detected by ELISA.40patients with head and neck squamous cell carcinoma (20patients with laryngeal squamous cell carcinoma and20patients with hypopharyngeal squamous cell carcinoma),20patients with precancerous lesions,20normal peoples were enrolled in this study. Software SPSS17.0is used for analyzing the results. We compared the difference of OPN and MMP-9expressions in the three groups with One-Way ANOVA;the differences of ages in the three groups with One-Way ANOVA;the expressions in different clinical stages, with and without lymph node metastasis with t-tests. P<0.05is regarded as the difference has statistical significance.Results:(1) The level of OPN and MMP-9in plasma of head and neck squamous cell carcinoma group is higher than normal group and precancerous group (P<0.05); There is significant difference between the level of OPN in plasma of precancerous group and that of the normal group (P<0.05), but the level of MMP-9in plasma between them has no significant difference (P>0.05).(2) The plasma level of OPN and MMP-9in lymph node metastasis group is higher than that without lymph node metastasis group (P<0.05). The plasma level of OPN and MMP-9aren’t correlated with the gender and age.(3) The level of OPN in plasma of HNSCC is correlated with that of MMP-9.Conclusion:(1) The level of OPN and MMP-9in plasma of patients with HNSCC can reflect the burthen of tumor.(2) OPN and MMP-9take part in tumor invasion and metastasis.(3) OPN and MMP-9in plasma of head and neck squamous cell carcinoma may cooperate with each other in happening and development of HNSCC.(4) OPN and MMP-9in plasma of patients with HNSCC can be considered as a maker of HNSCC and has greatly significance in monitoring of head and neck squamous cell carcinoma.