The Expression Of CK19 And E-cad In Peripheral Blood During The Tongue Mucosa Carcinogenesis

Objective:Oral cancer is the sixth in malignant tumors.Because of its high malignant degree and low survival rate,it is a serious threat to human health.Oral squamous cell carcinoma(OSCC)which is the most common oral malignant tumor accounts for more than 90%in oral cancers.The vast majority of squamous cell carcinoma of the oral mucosa has a definite precancerous damage phenomenon,so the study of different stages of disease and clinical changes is of important significance in the treatment of OSCC.Cytokeratin 19(CK19)is found in a variety of oral and maxillofacial lesions,which is a kind of cytoskeleton acidic peptide,including 40 × 103da molecular.Besides,it is a marker for epithelial cells characteristic,whose function will not lose with the cancer cells.Cytokeratin 19 is a type I keratin,mainly found in the epithelium of the epithelial cells of the simple epithelium or rapidly dividing cells.Previous studies just detected different differentiation degree of oral squamous carcinoma tissues and discussed the content of serum CK19,if the protein which can be used as the indicators of oral squamous cell carcinoma of is uncertain.E-cadherins is a kind of calcium dependent transmembrane protein,mainly mediated the same intercellular adhesion reaction.All kinds of calcium adhesive molecule have the common structural part,including extracellular region,transmembrane region,and region in the cell.At present,the research of scholars both at home and abroad indicate that E-calcium viscosity reduced in cancerous tissue expression,but the formation of cancerous by mild epithelial dysplasia to moderate epithelial dysplasia is not reported.This study intends to get whole data during the whole process of cancer development of E-cad and CK19.By analyzing the change of quantity in the process of cancer of the oral mucosa,this article aims at providing powerful evidence for the treatment of precancerous diagnosis and cancer prognosis.Methods:75 SD rats were administered 0.004%4-Nitroquinoline-1-oxide(4NQO)in drinking water from the age of 6 weeks to the age of 30 weeks,15 SD rats were kept in the same conditions without 4NQO,to act as negative controls.Killed the rats at different stages.Tumors were removed from the surrounding mucosa and cut into four pieces,one of which underwent histological examination,and the others were kept frozen.Determine the pathological classification and the nature of the lesion.Cases in peripheral blood of rats,according to the classification of the number of the subjects can be divided into:the control group,epithelial hyperplasia group,mild epithelial dysplasia group,moderate and severe epithelial hyperplasia group,tongue squamous cell carcinoma group.Used ELISA method strict inspection,purchase biological technology co.,LTD.Shanghai research domain to sustain rat CK19 were kits and E-cad kits,to sustain the rat CK19 were kit using double antibody rat clip method to determine the specimen cuhkkeratin.Made from purified rat keratin,solid phase antibody,the package in the sheet resistance of the microporous ordinal keratin,again with HR.P labeling keratin,type I cytoskeleton 19(krt19)antibody,the formation of antibodies-antigen-enzyme standard compounds,thoroughly and substrate TBM color after washing.The depth of the color and the sample of the keratin,I type cytoskeleton 19(krt19)were positively correlated.E-cad kit is solid clip art experiment enzyme-linked immunosorbent(ELISA).The concentration of E-known cad standard,the concentration of unknown sample into pores within the enzyme label plate for testing.Enzyme standard instrument under 450 nm wavelength determination of absorbance(OD value),via a standard curve to calculate samples under test concentration,so as to obtain the data needed.Results:In the experimental rats we got 82 valid subjects as well as 8 dead rats.In 82 valid rats:normal mucous membrane is 25.6%(21/82),tongue epithelial hyperplasia of pure 25.6%(21/82)17.1%(14/82),mild dysplasia,moderate dysplasia 9.8%(8/82),4 severely abnormal hyperplasia of 2.4%19.5%(2/82),squamous cell carcinomas(16/82).Experimental patholocgy classification results using hierarchical data of multiple independent samples Kruskal Wallis rank and inspection,tongue mucosa chi-square ?20.738,P<0.001;Palatal mucosal chi-square =15.003,P<15.003).The distribution differences,there is statistical significance.In 4 nqo water tongue SPF SD rats induced mucosal canceration process,CK19 were,E-cad in peripheral blood of rats with the increase of degree of abnormal epithelial its content are increased.CK19 were experimental group:CK19 were in control group,epithelial hyperplasia of simple groups,mild epithelial dysplasia,moderately severe hyperplasia and canceration group(control group,epithelial hyperplasia of simple groups,mild epithelial dysplasia group,relation between abnormal epithelial hyperplasia group,tongue squamous cell carcinoma group.With the full name,behind all this]the concentration of the average concentration are:593.45,784.45,848.58,784.45,848.58.In diff between groups:in the experimental group of CK19 were:CK19 were in the normal group,the simple hyperplasia group,mild dysplasia,concentration of moderately severe hyperplasia and canceration group average concentrations are:593.45± 134.06,784.45±108.53,848.58±99.68,951.94±85.31,1004.22±127.65.In the differences between the groups compare CK 19 were:In the experimental group and the rest of the group are the control group(P<0.01,according to the alpha = 0.05,the control group and epithelial hyperplasia of simple groups,mild epithelial dysplasia,the relation between abnormal epithelial hyperplasia,tongue cancer group differences were statistically significant;Epithelial hyperplasia of simple and mild epithelial abnormality group P= 0.113,there was no statistically significant difference in alpha = 0.05;Mild epithelial abnormality in the group and the degree of epithelial abnormality group P = 0.310,there was no statistically significant difference in alpha = 0.05;Cancer group and the rest of the group(P<0.05,according to the alpha = 0.05 difference was statistically significant,tongue cancer group and the control group and epithelial hyperplasia of pure group,mild epithelial dysplasia,the relation between epithelial dysplasia group differences were statistically significant.E-cad in the experimental group:E-cad in the normal group,the simple hyperplasia group,mild dysplasia,concentration of moderately severe hyperplasia and canceration group average are:259.92±89.75?402.85±93.52?412.39±64.08?466.25±66.72?539.14±108.07.In difference comparisons between groups:groups and the control group(P<0.01,according to the alpha =0.05,the control group and epithelial hyperplasia of simple groups,mild epithelial dysplasia,the relation between abnormal epithelial hyperplasia,tongue cancer group differences were statistically significant;Pure epithelial hyperplasia and mild epithelial dysplasia group P = 0.756,there was no statistically significant difference in alpha = 0.05;Pure epithelial hyperplasia group and the relation between abnormal epithelial hyperplasia group(P = 0.066,there was no statistically significant difference in alpha = 0.05;Mild epithelial dysplasia and the relation between epithelial dysplasia group between P = 0.146,there was no statistically significant difference in alpha = 0.05;Tongue cancer group and between groups(P<0.01,and according to the alpha = 0.05,the control group and epithelial hyperplasia of simple groups,mild epithelial dysplasia,the relation between abnormal epithelial hyperplasia,tongue cancer group differences were statistically significant statistical significance was found between A and B,and CK19 were E-cad in rat tongue mucosa of cancerous tissue expression in different stages of the relevance:CK19 were and E-cad in the different stages of the cancerous changes of rats were expression,expression rate in general are at a higher level,CK19 were positive staining cells are scattered in the base layer is distributed in the mucosa,basal upper don't express,E-calcium mucin exist in all epithelial tissue.They expressed in rat tongue cancer process conditions change are showing a rising trend.Cad the expression of CK19 were with E-with the increase of the degree of pathological changes,the rs = 1,p = 0.000,p<0.05,the link between positive correlation was statistically significant.Conclusion:(1)in 4 nqo water tongue process,E-in peripheral blood of cad content increases with the rise of histopathologic grading.The CK19 in oral squamous cell carcinoma in the expression and organization were positively associated with the degree of differentiation,which helps to diagnose the malignant degree of tumor tissue.E-reduced cad expression,tumor development,invasion and metastasis are closely related.(2)CK19 is showeded in peripheral blood of rats dental epithelial hyperplasia of pure and there was no significant difference between mild epithelial dysplasia,but there was a significant difference and relation between epithelial abnormalities.Whether it can be seen in the peripheral blood expression of CK19 is able to identify and the relation between abnormal hyperplasia can be used as a symbol of judgment before the oral lesions.(3)E-cad in the peripheral blood expression showed rats dental epithelial hyperplasia of pure and there was no significant difference between epithelial dysplasia.Whether you can think of E-cad expression in peripheral blood can't identify simple and abnormal hyperplasia as a symbol of judgment before the oral lesions are neither sensitive nor specific.(4)The quantity of CK19 can be used as a marker to predict the increase of the degree of pathological changes,and the link between positive correlations was statistically significant.