Analysis Of Colorectal Cancer Hematogenous Micrometastasis Between Laparoscopic And Open Radical Operation

Objective:The Laparoscopic technology has been used in surgeries for many years worldwide. In order to observe the effect of the laparoscopic radical operation in colorectal cancer (CRC), we detect pre-and post-operative micrometastases of laparoscopic and conventional Laparotomy radical operation to analyze whether there is difference between the two operations, and evaluate the clinical significance of laparoscopic resection operation in CRC.Methods:The peripheral blood samples were collected from patients with primary CRC at one day ahead and behind operation at The Third Affiliated Hospital of Kunming Medical College from March2012to December2012.40cases with laparoscopic resection and40cases with conventional Laparotomy were collected. Total RNA were extracted from karyocyte cells in the blood samples. CDX2mRNA and CK20mRNA were detected by FQ-PCR to compare the micrometastasis between laparoscopic and conventional Laparotomy radical operation.Results:In patients underwent and traditional open radical resection colorectal cancer, CDX2mRNApositive rate was80.0%(32/40) in laparoscopic resection and82.5%(33/40) in Laparotomy before operations; the positive rate was65.0%(26/40)in laparoscopic resection and72.5%(29/40)in Laparotomy a,fter operations.CK20mRNA positive rate was40.0%(16/40) in laparoscopic resection and37.5(15/50) in Laparotomy before operations; the positive rate was50.0%(20/40) in laparoscopic resection and45.0%(18/40)in Laparotomy after operations. There was no difference between pre-and post-operation (P>0.05). Compared CDX2mRNA and CK20mRNA expression with laparoscopic and conventional Laparotomy radical operation, there was no significant difference of the two groups (P>0.05).Conclusions:Compared withconventional Laparotomy radical operation, Laparoscopic radical resection operated in colorectal cancer doesn’t decrease the positive rate of CDX2mRNA and increase the positive rate of CK20mRNA. The results suggest that Laparoscopic radical resection doesn’t increase the risk of blood circulation micrometastasis of colorectal cancer cells.