Apoptosis Of Ovarian Cancer Cell And TRAIL Expression In TP Combination With TNF And IFN

[Objective] This study intended to use TP chemotherapy combined with cytokines TNF、IFN to increase TRAIL expression in ovarian cancer cells and the tumor cell apoptosis in order to improve the effect of chemotherapy. For TRAIL in clinical application to provide the necessary basis in the future, and explored a new treatment approache of high efficiency, small side effects, low resistance, eventually to improve the patients survival rate and life quality.[Methods] The patients from the department of gynecology of the third affiliated hospital of Kunming Medical University were chosen to this study with randomized, blinded. Grouping was as follows:group①latinum-based chemotherapy; group②Tumor necrosis factor (TNF) plus platinum chemotherapy; group③Interferon(γ-IFN) plus platinum chemotherapy; group④Interferon+Tumor necrosis factor Tumor necrosis factor plus platinum chemotherapy. Before treatment we got part of tumor tissue guided by ultrasonography, and put the tissue into tube, added a small amount of saline, then kept it in a-80℃refrigerator. The other part of the tumor tissue sent to pathological examination. We treated these patients with randomized after pathological diagnosis is "epithelial ovarian malignant tumor". We give these patients2routine chemotherapy according the four groups after they agreed this study, and signed on the book. Then3-4weeks after chemotherapy we collected the tumor tissue during surgery again. Using flow cytometry instrument detected ovarian cancer cell apoptosis, TRAIL and its death receptors, multi-drug resistance (MDR) gene expression between before and after treatment and observed curative effect and side effects.[Result] 1Comparison of before and after treatment in the each groups1.1Comparison of before and after treatment in the group①There was no significant difference in TRAIL and its death receptors、Tumor cell apoptosis rate between before and after treatment in the group①(P>0.05);1.2Comparison of before and after treatment in the group②There was significant difference in TRAIL、TRAIL death receptors and Tumor cell apoptosis rate between before and after treatment in the group②(P＜0.05);1.3Comparison of before and after treatment in the group③There was significant difference in TRAIL、TRAIL death receptors and Tumor cell apoptosis rate between before and after treatment in the group③(P＜0.05);1.4Comparison of before and after treatment in the group④There was significant difference in TRAIL、TRAIL death receptors and Tumor cell apoptosis rate between before and after treatment in the group④(P＜0.05);1.5There was significant difference in CA125and volume of tumor between before and after treatment in the each groups (P＜0.05); There was no significant difference in MDR between before and after treatment in the each groups (P＞0.05)2Comparison of each groups2.1TRAIL The TRAIL expression rate in group④was higher than other groups. There was a significant difference between group④and other groups (P＜0.05);2.2TRAIL death receptors There was a significant difference between group①、 group②and group③、group④(P＜0.05);2.3apoptosis of Ovarian cancer cell There was a significant difference between group①、group②and group③、group④(P＜0.05);2.4MDR:there was no significant difference between four groups (P＞0.05);2.5CA125:There was a significant difference between group④and other groups (P＜0.05);2.6volume of tumor:there was a significant difference between group④and other groups (P＜0.05);2.7The side effects of chemotherapy:The most common side effects was bone marrow suppression Ⅰ°and Ⅱ°, Liver and kidney function damaged were transient, a period of time after Chemotherapy, its could be improved after treatment.3The relationship between the indicators: 3.1The TRAIL and death receptors had a linear relationship; As the expression of receptors increased when TRAIL expression increased. The TRAIL and apoptosis of Ovarian cancer cell had a linear relationship; As the rate of Ovarian cancer cell apoptosis increased when TRAIL expression increased.3.2The TRAIL and MDR had a negative correlation; As the expression of TRAIL increased, The expression of MDR declined.3.3The TRAIL and CA125had no correlation.[Conclusion]1. TP combination with TNF, IFN could obviously increase the TRAIL expression;2. TP combination with IFN could obviously increase the TRAIL death receptors expression;3. TP combination with IFN could obviously increase the rate of ovarian cancer cell apoptosis;4. TP combination with TNF, IFN could obviously reduce the CA125;5. TP combination with TNF, IFN could obviously reduce the tumor volume;6. TP combination with TNF, IFN caused no seriously side effects of chemotherapy;7. TP combination with TNF, IFN may be reduce the MDR;...