The Expression And Significance Of Fascin-1in Breast Carcinoma

Objective: To investigate the expression of Fascin-1(FSCN1) protein inparaffin-embedded human breast cancer and benign diseases, and its effect on breastcancer cells proliferation, invasion and migration potential.Methods: One hundred and twenty-four cases of breast carcinoma and27cases ofbenign breast lesions were analyzed by immunohistochemical staining. RNAinterference was used to inhibit FSCN1expression in two human breast cancer cell lines,MCF-7and MDA-MB-231. Cell proliferation of two kinds of cancer cells wasevaluated by MTT assay. Invasion and migration potential of MDA-MB-231cells wereevaluated by transwell invasion assay and transwell migration assay, respectively.Results:1. There was no significant difference of FSCN1protein expression between breastcancer and benign breast diseases (P＞0.05). The expression of FSCN1protein wassignificantly correlated with histological grading, ER and PR status and mitotic count (P＜0.05), but not with age, tumor size, axillary lymph node metastasis, TNM stage andc-erbB-2expression. The overall survival rates in FSCN1protein positive patients andnegative patients were78.7%and85.7%, respectively (P＞0.05).2. The expression level of FSCN1mRNA was significantly decreased in MCF-7transfected with FSCN1siRNA (P＜0.05). The protein expression level was alsomarkedly reduced in MCF-7transfected with FSCN1siRNA. 3. MCF-7and MDA-MB-231cells showed growth inhibition in FSCN1siRNAtransfectant compared with the control transfectant (P＜0.05) detected by MTT assay.4. Transfecting of FSCN1siRNA into MDA-MB-231cells led to a significant cellinvision and migration inhibition (P＜0.01and P＜0.05, respectively) detected bytranswell invasion and migration assay.Conclusion: FSCN1might positively regulate proliferation, invasion and migration ofbreast cancer cells and might be a potential biomarker candidate for breast carcinoma.