Experimental Study Of Nerve Growth Factor Mixed With The Scaffold Materials Repair Rabbit Mandible

Objective Bone tissue defects caused due to injury, cancer, infection and otherreasons, fora long time, has been the problems faced by the medical profession, andincreased patient morbidity, significantly affect the quality of life. The experimentthrough the production of rabbit mandibular bone defect animal models, and loadobserved exogenous nerve growth factor in the gelatin sponge and form a complex withthe scaffold material CERASORB bone meal, the the rabbit mandibular repair process,application of imaging and statistical methodsanalysis and evaluation, research topicalapplication of nerve growth factor composite scaffold for therabbit mandible rippedthrough the bone defect new bone formation affect NGF composite scaffold for bonedefect healing effect to provide experimental evidence. For the clinical treatment ofbone defects, bone nonunion, and alveolar bone resorption, reduce patient morbidityand improve quality of life, and may providefurther clinical problems.Methods18adult New Zealand rabbits were randomly divided into three groups,the experimental group, the positive control group and negative control group, allanimals were mandibular body size15mm×8mm preparation unilateral rippedthroughthe bone defect model, the experimental group plantingcoverage after the theappropriate amount CERASORB bone meal to NGF gelatin sponge. With the droppingof the gelatin sponge with normal saline control group the implant right amount ofCERASORB bone meal after covering. Comparison group did not implantany material,layered suture the wound dressing daily intramuscular injection of penicillin to prevent infection. Were two weeks, four weeks, eight weeks in eachgroup, two animals weresacrificed to obtain samples, gross observation, radiological observation, and theobserved results were statistically analyzed. Analysis of observational studies of bonedefects different experimental process (2,4, and8weeks) tissue defects injurycompared.Results1.The postoperative laboratory animals no obvious signs of infection, wound healingsurgery area, no significant effusion occurred.2.After2weeks: group periosteal reaction. The negative control group, showingtheformation of a small amount of bone and cartilage defects around, shows a smallamount of granulation tissue within the defect. Positive control group bone defect bonemeal are linked closely together, a large number of new granulation basic and cartilagetissue to fill the defect area finger touched Organization tough, not easy to exfoliation.Experimental group, bone meal and bone tissue is linked closely together, dense softtissue surrounding the bone defect area, seen aftera large amount of granulation and softtissue in the defect area, fibrous tissue avulsion. X-ray observation: the groups were noobvious new bone formation, thesharp edges of the bone defect site, the experimentalgroup and the positive control group, the X-ray visible defects, bone meal.4weeks after surgery: negative control group bone defect healing wasacceptable,dense soft tissue filling the defect area, reduce the scope of the bone defect.The positive control group bone defect range relative to the negative control groupfurther reduced, bone meal and soft tissue defects are linked closely together.Experimental group area basic healing of bone defects, visible defects around a lot ofhard and soft tissue coverage. X-ray observation: each group were seen varying degreesof osteoblastic reaction, the defects of the experimental group, bone meal basicabsorption, showing that a small amount of residual absorption of bone meal, the defect obviously see the formation of new bone shadow, and a large range of accounting forthe defect area1/2. The positive control group showed bone powder substantiallyabsorbs the remaining about1/3of the bone meal is not absorbed at the edges Moviessmaller range of apparent new bone formation, only the vicinity of the edge is visible.The negative control group showed new bone formation reaction, a small range, onlythe edge of a small amount into the bone defect shadow. About1/5of the defect area.After8weeks: the healing of bone defects in good condition. The experimentalgroup has healed bone defects, the original defect range surface are still not fully absorbbone meal. Positive control group bone defect healing relative experimental groupdifference defects healing, no significant ripped through the missing gap, the originaldefect range can be seen more unabsorbed bone meal. The negative control group notyet completely healed, more soft tissue defect within. X-ray observation: theexperimental group showed healing of bone defects, defects of the central part of thebone mineral density is slightly lower, visible bone defects within clear trabecularstructure shadow, only a small amount of bone meal particles were not completelyabsorbed. The positive control group bone defect bone healing, old and new bone at thejunction of the gap is not completely absorbed, scattered in a small amount of bonemeal particles. The bone defects most of the negative control group healing, unevendensity, the boundary line of the old and new osteoblasts obvious.3. Compare micro CT the observed value: bone mineral density (BMD), relative bonevolume (BV/TV), trabecular number (Tb.N), the experimental group than the controlgroup and the difference was statistically significant (P <0.05). Surface area to volumeratio (BS/BV), the experimental group than the control group, but the difference wasnot statistically significant (P>0.05). These results further support the specimens andX-ray examination.Conclusion Nerve growth factor gelatin sponge composite CERASORB bone meal in mandibular defect repair time is significantly shorter than the two control groups, basicand complete bone repair,8weeks after surgery prompted gelatin sponge as a carrier ofNGF composite CERASORB of bone meal scaffolds significantly promote bonedefects,has good biocompatibility and operability.