The Influence Of MicroRNA-429on The Proliferation And Invasion Of Human Bladder Cancer Cells

Objectives：To explore the effecting mechanism of miR-429in human bladdercancer cells through observing the influence of miR-429on the proliferation andinvasion of human bladder cancer BIU-87cells.Methods： First, differentially expressed miRNAs of normal tissue adjacent tohuman bladder cancer tissue were selected with the help of the gene chip, and theirdifferential expressions were tested by real-time quantitative PCR. Then, a miR-429antisense nucleotide sequence（AS-miR-429）was constructed, miR-429expressionwas down-regulated and the sequence was transiently transfected into human bladdercancer BIU-87cells. Furthermore, the change of miR-429expression level wasdetected by real-time quantitative PCR. Finally, the cell proliferation and apoptosiswere detected by MTT test and flow cytometry instrument, and cell aggressivity wasdetected with the Trans-well test.Results： Gene chip screening found the differential expressions of miR-429,miR-143, and miR-106a in the bladder cancer tissue, including miR-429significantlyup-regulated. Real-time quantitative PCR also proved that miR-429was significantlyup-regulated in bladder cancer tissue. A miR-429antisense nucleotide sequence wassuccessfully constructed, and was transiently transfected into human bladder cancerBIU-87cells. The result of transfection by real-time quantitative PCR showed thatmiR-429expression decreased by16times in bladder cancer cells and the differencewas statistically significant (P <0.05). Forty-eight hours after transfection, MTTdetection found AS-miR-429significantly inhibited the proliferation of BIU-87cells.Flow cytometry instrument was used to assay the apoptosis rate of BIU-87cell, whichincreased significantly. While the result of Trans-well test indicated that AS-miR-429had obvious influence on the aggressivity of BIU-87cells.Conclusions：1.miR-429was significantly up-regulated in bladder cancer tissue.2.miR-429promoted the proliferation of bladder cancer cells, and increased the aggressivity of bladder cancer cells significantly.