Gubi Mixture Effect For Collagen TypeⅡ.MMP-13in The Cut The Double Forelegs Induce The Rats To Up Joint Hulth Method Modle Of The KOA Knee Osteoarthritis Model In Rats

Objective: to establish a kind of similar to human disease process to double Hulthforelegs vertical joint improvement method of KOA knee osteoarthritis in rats model,observe the affect by cut the double forelegs in rats, research the influence pass cutthe forelegs induce the rats to up joint Hulth method to build the KOA kneeosteoarthritis model in rats. To move forward a single step research cartilagoarticularis regression mechanism. And with this model as the research tool.Research the Gubi mixture effect that delay effect of the articular cartilagedegeneration.Method:Choose only10days of age48SD rats, Were randomly divided into normalcontrol group. cut the double forelegs induce the rats to up group. cut the doubleforelegs induce the rats to up joint fake Hulth method modle group.cut the doubleforelegs induce the rats to up joint Hulth method modle group. Total4groups (eachgroup of12). normal control group not to cut double forelegs, The rest of the threegroups is cut the double forelegs. Raised in cage breeding.the group is fake Hulthsurgery in the cut the double forelegs induce the rats to up joint fake Hulth methodmodle group four months after The first time surgery. the group is Hulth surgery inthe cut the double forelegs induce the rats to up joint Hulth method modle group fourmonths after The first time surgery. In the whole experiment process. Measuring therat body weight, body length, tail length, tail, right foot long for every once in amonth. After the second time surgery, Abdominal cavity anesthesia based in the four group. Measuring the volume of lower limbs through cut double lower limbs from Inthe groin. Obtain the four sets of knee joint organization respectively for HE stainingand toluidine blue staining and hiding the red solid green staining of Collagen typeⅡ (Collagen Ⅱ), Matrix Metalloproteinas-13(MMP-13) immunohistochemicalstaining.Choose only10days of age52SD rats,all rats is cut the double forelegs.Raised in cage breeding. the four group is Hulth surgery,four months after The firsttime surgery. One month after the second operation,the four group were randomlydivided into Blank control group, i Gubi mixture group, Glucosomine AndIndomethacin erterucoated Tablets group, normal saline group (13in each group).Gubi mixture group give Gubi mixture0.5ml/100g According to the weight,Glucosomine And Indomethacin erterucoated Tablets group give Glucosomine AndIndomethacin erterucoated Tablets0.5ml/100g (Soluble in the same volume ofphysiological saline)According to the weight, normal saline group give normalsaline0.5ml/100g According to the weight, Blank control group don’t intervene.Continuous lavage for4weeks, Anesthesia is executed for each group of rats, obtainthe knee joint for HE staining and toluidine blue staining and hiding the red solidgreen staining of CollagenⅡ,MMP–13immunohistochemical staining.Results:Grow well in rats n the process of the first part of the experiment, in all48rats, only one death, Regularly measure the rat body weight, body length, tail length,tail, right foot long, Between2months to5months, Normal group rats weight andlower volume than with the other three groups has statistics difference, the Hierarchystructure destruction of cartilage, cartilage, cartilage layer can see some holes in thecut the double forelegs induce the rats to up joint Hulth method modle group forHE staining and toluidine blue staining and hiding the red solid green staining,can’tsee the Surface layer, transition layer, radiation layer and calcified layer and thebonding line in the all Structure of articular cartilage. In the articular cartilage, cansee a lot of hypertrophy of cartilage cells, Cartilage level already can’t see, the tideline could not see, the uneven dyeing of articular cartilage, including dyeing andcartilage layer, almost calcified layer, it is obvious degeneration of articular cartilage. Collagen Ⅱimmunohistochemical display CollagenⅡ positive cells mainlydistributed in deep cartilage, uneven, content is significantly reduced in the cut thedouble forelegs induce the rats to up joint Hulth method modle group. MMP-13immunohistochemical experiments show that MMP-13positive cells statistics ishigher than other three groups in the cut the double forelegs induce the rats to upjoint Hulth method modle group.Grow well in rats n the process of the first part ofthe experiment, in all48rats, only two death, Cartilage surface layer, transition layer,radiation layer, calcified layer and the bonding line can see some, some unevendyeing, articular cartilage surface cells gathered, the transition layer of cartilage cellsarranged disorderly, see some features of cartilage cells, cartilage cells increased, theradiation layer cells arranged columnar, tidal line is complete, the hypertrophy ofcalcified layer visible cartilage cells for HE staining and toluidine blue staining andhiding the red solid green staining in the Gubi mixture group. Gubi mixture groupcompared with blank control group Collagen Ⅱ immunohistochemical displayCollagen Ⅱ the number of positive cells increased obviously. Gubi mixture groupcompared with the other three groups,MMP-13immunohistochemical experimentsshow that MMP-13the number of positive cells were lower than other three groups.Conclusion:cut the double forelegs induce the rats tu up can chang the Force of thearticular cartilage of knee joint and surrounding tissue. In the joint Hulth methodbase can Successfully established the KOA knee osteoarthritis modle. Whether fromthe point of common dyeing, special, or through the immune inhibitor, this buildingmethod can increase the force of the knee joint, ultimately affect the articularcartilage cells, broke the metabolic balance of articular cartilage cells, decreasedarticular cartilage cells secrete Collagen Ⅱ ability, increase the cartilage cells abilityof MMP-13, the final structure is articular cartilage extracellular matrix by MMP-13decomposition, especially Collagen Ⅱ break down faster and accelerated thedegeneration of articular cartilage, eventually causing model of kneeosteoarthritis.Gubi mixture can impact on knee joint osteoarthritis model by cut thedouble forelegs induce the rats tu up joint Hulth method. Whether by HE stain, toluidine blue staining and hiding the red solid green dyeing, or Collagen Ⅱ, MMP-13immune resistance test results, involving the bone mixture can promote secretionof articular cartilage Collagen Ⅱ, increase the content of extracellular matrix,reducing the release of MMP-13, delay the degradation of the extracellular matrix,especially Collagen Ⅱ degradation, delay the degeneration of articular cartilage ofknee joint process.