| Objective: through HE+ alcian blue and the double staining, toluidine blue staining and safranin fast green staining histomorphological observation and immunohistochemistry of method for the detection of Fas and FasL gene expression,study the bone rheumatism) mixture in the treatment of part of the influence mechanism of knee osteoarthritis model in rats. The correlation between clinical investigation of knee osteoarthritis of different X-ray grading and hip arthritis.Method:the experimental part: the KOA model of the bilateral anterior limb upright combined with Hulth method is a simple, reliable, high success rate, similar to the human KOA pathogenesis of animal KOA model [1-4]. SD rats were used during pregnancy 15 days only 12, the average distribution in different cages. The pups born to be. Pups born on the 10 th day, the 80 mice, sort of body weight were randomly divided into four groups according to: blank control group(to double forelegs are straight with Hulth method rat koa model group, saline group, glucosamine indometacin group, Gubi Decoction group, with 20 rats in each group, in each group was then divided into two groups respectively for 6 months and 9 months of age. All the experimental rats were intraperitoneal anesthesia after surgery to remove the double forelimbs, put in cages. In April, two groups were removed after operation for Hulth forelimb right knee surgery. In June, aged rats were given drug interventionafter second times of operation in January. Blank control group SD rats were given normal diet; the normal saline group SD rats in addition to the normal diet, the normal saline 10mL/kg/d for filling the stomach, points 8 points, 18 points each 1 times.Glucosamine indometacin group SD rats except the normal diet and treated with glucosamine indometacin enteric coated tablets 10 mg / kg / D, placed in the same volume of distilled water to be melted shake after 8 o’clock, 18 points each time by gavage. In addition to the normal diet, SD rats were treated with 10mL/kg/d, 8 points,18 points each for 1 times, and 4 weeks for the intervention process. In September,aged rats were given drug intervention after second times of operation in April. The process of drug intervention and the duration of the same age group of rats in June.Respectively in the two time of 6 months, 9 months and in the peritoneal cavity after successful anesthesia were experimental rats and of total knee joint were collected,the knee joint after fixation and decalcification, dehydration, paraffin embedded preparation wax specimens, paraffin blocks through slicing, the exhibition, fishing tablets, toast and embedded in paraffin wax, on paraffin sections of special staining,including: HE+ alcian blue and counterstained, stained with toluidine blue and safranin solid green staining. Under the optical microscope to observe each knee joint histomorphology feature and select the specific parts of the film and histological observation at the same time, each rat knee cartilage tissue by the Mankin score standard score, statistical analysis. Also on paraffin sections by immunohistochemistry of detection technology of double forelegs are straight with Hulth method rat osteoarthritis of the knee arthritis model groups of knee joint of Fas and Fas L gene expression in ovariectomized, optical microscope under the same multiples of vision to observe and count each of the knee positive cell number, and select specific parts of the picking film, using SPSS17.0 statistical software was used to statistic and analysis concluded. Clinical part: through the questionnaire will meet the inclusion criteria of knee osteoarthritis and(or) hip joint inflammation in patients with X-ray classification, physical examination and fill in the questionnaire, the questionnaire data were collected, the results were statistically analyzed using SPSS17.0 statistical software.using chi square test, Spearman rank correlation coefficient tomeasure the correlation between X-ray grade and the incidence of hip joint disease in patients with osteoarthritis of the knee.Results:1.from a variety of special staining of histological observation after that: no matter at the age of 6 months or 9 months of age, and other groups(blank control group, saline group, glucosamine indometacin group) the lightest contrasts cartilage cell degeneration of is Gubi Decoction group.2. 6 month old bone Tongbi mixture group and also three groups(blank control group,saline group, glucosamine indometacin group)(P < 0.01), that there is a significant difference between the Gubi Decoction group and other three groups; 6 month old articular cartilage Mankin score is the lowest bone Tongbi mixture group, with a value of 50. X=5.0 So it is considered that the degeneration of articular cartilage in 6 month old bone Tongbi mixture group than the other three groups(the blank control group,saline group, glucosamine indometacin group) light, Gubi mixture can delay the development of KOA lesions.3.and 9 months of age Gubi Decoction group and also three groups(blank control group, saline group, glucosamine indometacin group)(P < 0.01), that there is a significant difference between the Gubi Decoction group and other three groups; 9month old articular cartilage Mankin score is the lowest bone Tongbi mixture group,worth a total of 68, X=6.8 So it is considered that the degeneration of articular cartilage in the 9 month old bone Tongbi mixture group than the other three groups(the blank control group, saline group, glucosamine indometacin group) light, Gubi mixture can delay the development of KOA lesions.4.6 month old bone Tongbi mixture group and other three groups(blank control group,saline group, glucosamine indometacin group)(P < 0.01), that there is a significant difference between the Gubi Decoction group and other three groups; 6 month old articular cartilage of Fas positive cells expression is the lowest bone Tongbi mixture group. So it is considered that the degeneration of articular cartilage in 6 month old bone Tongbi mixture group than the other three groups(the blank control group,saline group, glucosamine indometacin group) light, Gubi mixture can delay thedevelopment of KOA lesions.5.and 9 months of age Gubi Decoction group and also three groups(blank control group, saline group, glucosamine indometacin group)(P < 0.01), that there is a significant difference between the Gubi Decoction group and other three groups; 9month old articular cartilage of Fas positive cells expression is the lowest bone Tongbi mixture group. So it is considered that the degeneration of articular cartilage in the 9month old bone Tongbi mixture group than the other three groups(the blank control group, saline group, glucosamine indometacin group) light, Gubi mixture can delay the development of KOA lesions.6.6 month old bone Tongbi mixture group and other three groups(blank control group,saline group, glucosamine indometacin group)(P < 0.01), that there is a significant difference between the Gubi Decoction group and other three groups; 6 month old articular cartilage Fas L positive cells expression is the lowest bone Tongbi mixture group. So it is considered that the degeneration of articular cartilage in 6 month old bone Tongbi mixture group than the other three groups(the blank control group,saline group, glucosamine indometacin group) light, Gubi mixture can delay the development of KOA lesions.7. 9 month old bone Tongbi mixture group and other three groups(blank control group, saline group, glucosamine indometacin group)(P < 0.01), that there is a significant difference between the Gubi Decoction group and other three groups; 9month old articular cartilage Fas L positive cells expression is the lowest bone Tongbi mixture group. So it is considered that the degeneration of articular cartilage in the 9month old bone Tongbi mixture group than the other three groups(the blank control group, saline group, glucosamine indometacin group) light, Gubi mixture can delay the development of KOA lesions.8. 321, in accordance with the inclusion criteria and complete the survey of 6 patients,the incidence of male patients was significantly lower than that of female patients,about female patients with 1/4; the incidence of patients with the elderly mainly; the proportion of the Han nationality is 98.75%. Gender, age, weight, history of trauma,and surgical history may be the factors that induce knee osteoarthritis. Results theclinical risk factors and characteristics of KOA were consistent with the results;9.through statistical analysis of specialist diagnosis, osteoarthritis of the knee X-ray classification, the location of the cancer, that patients with knee osteoarthritis in most of the double knee disease, osteoarthritis of the knee and hip osteoarthritis were onset phenomenon; in patients with knee osteoarthritis(OA) of the knee X-ray classification, diseased parts without obvious specificity, around the knee incidence rates and around the knee X-ray grading proportion had no significant difference.10. the koa patients X-ray grading and left and right hip pain, stiffness, sense of time,the flexion function and x-ray report results, the left and right leg Alice legs function correlation analysis can see: osteoarthritis of the knee X-ray different grading and left and right hip pain, flexion function, x-ray report results, left and right leg curling legs function correlation exists; the left and right hip joint stiffness and sense of time does not exist between the. Therefore, it is considered that osteoarthritis of the knee arthritis X-ray grading and hip pain, flexion function, x-ray report results, left and right leg curling legs function symptom correlation exists; the left and right hip joint stiffness and sense of time of onset symptoms relevant.Conclusion:1.whether at the age of 6 months or 9 months bone age Tongbi mixture group and other groups(blank control group, saline group, glucosamine indometacin group), the degeneration of articular cartilage morphology is the lightest, Mankin score is the lowest bone Tongbi mixture group, the bone Tongbi mixture can delay koa lesion development.2.whether June or September old bone rheumatism mixture may be reduced by Fas,Fas L gene expression mechanism to delay the development of KOA lesions.3. the incidence of male patients was significantly lower than that of female patients,about 1/4 of female patients; the incidence of patients with the elderly mainly; the proportion of the Han nationality is 98.75%. Gender, age, weight, history of trauma,and surgical history may be the factors that induce knee osteoarthritis. Results the clinical risk factors and characteristics of KOA were consistent with the results;4.with osteoarthritis of the knee in most of the double knee disease, osteoarthritis ofthe knee and hip osteoarthritis were onset phenomenon; there were no obvious specificity in patients with knee osteoarthritis(OA) of the knee X-ray classification,incidence of part of the right and the left, around the knee incidence rates and around the knee X-ray grading proportion had no significant difference.5.osteoarthritis of the knee X-ray different grading and left and right hip pain, flexion function, x-ray report results, left and right leg curling legs function correlation exists;the left and right hip joint stiffness and sense of time there was no correlation.Therefore, it is considered that osteoarthritis of the knee arthritis X-ray grading and hip pain, flexion function, x-ray report results, left and right leg curling legs function symptom correlation exists; the left and right hip joint stiffness and sense of time of onset symptoms relevant.in patients with osteoarthritis of the knee, the severity of the symptoms of the hip and the severity of the symptoms of knee joint symptoms of a positive correlation, the same side of the hip than the other side of the hip. |
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