Detection Of Gingipian Gene Of Porphyromonas Gingivalis In Atherosclerotic Plaque

Objective:The purpose of this study was to detect two genes （kgp-cd andrgpB-cd） of Porphyromonas gingivalis in atherosclerotic plaque from the patients withperiodontitis who scheduled for coronary artery bypass surgery，and determine therelationship between the two genes and the periodontal clinical indexes.Methods:45atherosclerotic plaque samples from the patients with periodontitis whoscheduled for coronary artery bypass surgery were included in this study. The extractedDNA was amplified with the primers designed to obtain the genes encoding the catalyticdomain of kgp and rgpB，and typing P.gingivalis into two genotypes.The relationshipbetween different genotypes of P.gingivalis and the periodontal clinical indexes wasstatistically analyzed by SPSS11.5software package for Students test and rgp-B，andthping P.gingivalis into two genotypes.The relationship between different genotypes ofP.gingivalis and the periodontal clinical indexes was statistically analyzed by SPSS11.5soteware package.Results:The detection rate of type-A P.gingivalis was higher than that of type-BP.gingivalis.Both prebe depth and bleeding on probing had significant difference betweendifferent genotypes of P.gingivalis.Supperted by National Natural Science Foundation ofChina and Natural Science Foundation of Liaoning Province.Conclusions:The presence of periodontal bacteria P.gingivalis in coronaryatherosvlerotic plaques could indicate that periodontal pathogenic bacteria may play a rolein the coronary heart disease process.