The Role And Upstream Regulation Of BCL-6on Proliferation, Invasion And Migration Of Human Breast Cancer Cells

Objective: To explore the role and upstream mechanism of BCL-6gene on proliferation,invasion and migration on human breast cancer cells.Methods:1. The expression of BCL-6mRNA in two strains of normal breast cells and six strainsof breast cancer cells was detected by qRT-PCR. Two breast cancer cell lines (highor low expression of BCL-6) were used for further study.2. MTT, Soft Agar and Transwell Assays were used to detect the functions of BCL-6on cancer cell proliferation, invasion and migrasion.3. Nude mice were used to detect the ability of xenograft formation in vivo.4. Bioinformatics tools and luciferase reporter assay were used to analysis and predictthe potential target miRNAs of BCL-6.5. qRT-PCR and western-blot were used to observed mRNA and protein expressions ofBCL-6by transfection of potential miRNA mimics to T47D cell.Results:1. The results of qRT-PCR showed that the expression of BCL-6mRNA in six breastcancer cell lines were higher than that in two normal breast cells (P<0.05). Twokinds of breast cancer cells (T47D and MCF-7) were used to further study. 2. The results of MTT, Soft Agar, Transwell assays showed that BCL-6has thepotential to promote breast cancer cells proliferation, non-anchored cell growth,invasion and migration.3. Nude mice tumor-bearing assay demonstrated that stable expression of BCL-6breast cancer cell of MCF-7had the potential to promote the ability of breast cancerxenograft formation (P<0.05).4. Bioinformatics tools and luciferase reporter assay demonstrated miR-339-5p as thetarget miRNA of BCL-6.5. The expression levels of BCL-6mRNA and protein were significantly decreased inT47D cell with forced expression of miR-339-5p.Conclusion:1. BCL-6gene may significantly promote the proliferation, invasion and migration ofbreast cancer cells and may be a valuable molecular marker for breast cancer.2. MiR-339-5p can regulate the expression of BCL-6in breast cancer cell.