Expression Of Fascin And E-cadherin、β-catenin In Intrahepatic Cholangiocarcinoma And Its Clinical Significance

Objective To investigate the expression level of Fascin,E-cadherin,β-catenin in intrahepatic cholangiocarcinoma and benign bileduct tissue, Statistical analysis of their relationship withclinicopathological parameters and prognosis,the relationship among theproteins. To investigate expression of Fascin,E-cadherin and β-catenin inintrahepatic cholangiocarcinoma and its clinical prognostic significance.Methods To observe the expression of Fascin, E-cadherin,β-catenin in62samples and20benign bile duct tissues by an immunohistochemicalmethod.There were5ICC tissues and5benign bile duct Fresh specimentissues,To observe the expression of Fascin, E-cadherin,β-catenin by awestern method．methods retrospective analysis was conducted on thedata of operation and follow up of62cases.The chi-square test,t test andthe Spearman rank correlation test analysis were adopted in statistics, theclinical significance of the protein expression was evaluated by statisticalanalysis. Results (1) Results of immunohistochemistry showed that thepositive expression rates of Fascin were significantly higher in the tumortissues than in benign bile duct tissue，Results there was significantdifference （P＜0.01）. The negative expression of E-cadherin and β-catenin were significantly higher in the tumor tissues than in benign bileduct tissue，Results there was significant difference （P＜0.01）.(2) Results of western blotting showed that the expression level ofFascin were significantly higher than that of controls(P<0.01)； Theexpression of E-cadherin、β-catenin significantly decreased in ICC thanthe control’s(P<0.01).(3) The expression of Fascin protein related to the histological grade，lymphaden metastasis and portal Vein invasion,intrahepaticcholangiocarcinoma associated hepatolithiasis(P＜0.05). E-cadherinrelated to the differentiated degree,metastasis through lymph node(P＜0.01).The relationship between negative expression rates of β-cateninprotein and clinical data including the differentiated degree,metastasis oflymph node,portal vein invasion and hepatitis virus(P＜0.05).(4) Kaplan-Meier methods were used to compute the survivalanalyses. The patients with positive Fascin expression had a significantlyshorter survival rate than those with negative expression (P＜0.05). Theexpression of E-cadherin was no significant difference between positiveand negative expression (P>0.05). β-catenin negative cases had a significantly poorer prognosis when compared with theβ-catenin positivecases (P<0.05).COX proportional hazard model multivariate analysisshowed that the key factors that influence the prognosis of ICC areFascin, β-catenin and the histological grade(P＜0.05).(5) Negative correlation between Fascin and E-cadherin (r=-0.451，P＜0.01). negative correlation between Fascin and β-catenin(r=-0.232，P＜0.01). but the positive correlation between E-cadherin and β-catenin(r=0.341，P＜0.01).Conclusions The expressions of Fascin in patient with malignanttumour were significantly higher than those in benign tumor patient，theexpression level of E-cadherin andβ-catenin in ICC tissues wassignificantly lower than that in matched benign tissues;Fascin and β-catenin are related with the prognosis of the patients with ICC;The co-expression pattern of Fascin, E-cadherin and β-catenin in ICC suggestthat they may act synergistically in development and progression.