Effects Of MiR-711on The Invasion, Metastasis And Proliferation Of Gastric Cancer Cell SGC-7901

objective: To investigate the effects of miR-711on the invasion, metastasis andproliferation of human gastric cancer cell SGC-7901by Transwell invasion test, cellscratch test and MTT test respectively, and to provide theoretic foundations for theprevention and treatment of miR-711on gastric cancer.Methods: miR-711mimics, miR-711inhibitors and miRNA empty plasmids weretransfected into human gastric cancer cell strain SGC-7901by liposomes transienttransfection method. miRNA empty plasmids transfection group was taken as annegative control group (NC group), and a blank transfection group taken as blankcontrol group (control group). In48h after transfection, the transfection effectivenesswas observed under a fluorescence microscope, and the post-transfection expressionof miR-711in each group was validated by Real-time PCR. The invasion ability ofthe cell was tested by Transwell invasion test, the metastasis ability of the cell testedby cell scratch test and the proliferation ability of the cell tested by MTT test.Results:(1) In48h after transfection, the cells were observed and counted under afluorescence microscope, and the effectiveness of miR-711mimics, miR-711inhibitors and miRNA empty plasmids transfecting human gastric carcinoma cellSGC-7901was above75%. The relative expression volumes of miR-711ofdifferent transfection groups were tested by Real-time PCR, and the expressionvolumes of miR-711mimics transfection group, miR-711inhibitors transfectiongroup and NC group were7658.6times,0.35time and1.2times of that of controlgroup respectively.(2) As revealed by Transwell invasion test (in48h after transfection, namely in24hafter microbiomation, the cells were observed under lower power lens*100):1)the number of invaded cells of the control group was39.5±1.9, while that of the miR-711mimics transfection group was12.1±1.4, and the later was significantlylower (P<0.05);2) the number of invaded cells of the miR-711inhibitorstransfection group was53.5±2.5, and the difference in the number of invaded cellsbetween the miR-711inhibitors transfection group and the control group wassignificant (P<0.05);3) the number of invaded cells of miRNA empty plasmidstransfection group was33.5±2.1, and the difference in the number of invaded cellsbetween the miRNA empty plasmids transfection group and the control group wasstatistically insignificant (P>0.05).(3) As revealed by the cell scratch test: In24h after scratch, the healing rate ofSGC-7901cells of the miR-711mimics transfection group was23.8%±3.2%,while that of the control group36.5%±2.6%, and the difference was significant(P<0.05); the healing rate of SGC-7901cells of the miR-711inhibitors group was48.7%±4.5%, and the difference between the miR-711inhibitors group and thecontrol group was significant (P<0.05). In48h after scratch, the healing rate ofSGC-7901cells of the miR-711mimics transfection group was56.2%±3.5%,while that of the control group76.3%±2.5%, and the difference was significant(P<0.05); the healing rate of SGC-7901cells of the miR-711inhibitors group was88.7%±4.3%, and the difference between the miR-711inhibitors group and thecontrol group was significant (P<0.05).(4) As revealed by MTT test, in48h,72h and96h after SGC-7901cells weretransfected with miR-711mimics (namely,24h,48h and72h after innoculation),the proliferation inhibition ratios were time-dependently increased, and were7.2%,8.3%and10.7%respectively; when compared with those of the control group, allthe differences were significant (P<0.05). In48h,72h and96h after SGC-7901cells were transfected with miR-711inhibitors, the proliferation ratios weretime-dependently increased, and were6.5%,8.6%and9.3%respectively; whencompared with those of the control group, all the differences were significant(P<0.05). The differences between the control group and the NC group wereinsignificant (P>0.05). Conclusion: miR-711mimics can inhibit the invasion, metastasis andproliferation of human gastric cancer cell SGC-7901, suggesting that miR-711isrelated with the onset and development of gastric cancer.