The Novel Methods Of Health Laboratory Technology For Methyl Methacrylate, Bisphenol A And Hydrogen Sulfide

Chapter1: The significance and headways trend of the health laboratorytechnologies were introduced respectively for methyl methacrylate,bisphenol A andhydrogen sulfide.The basic principles and the basis of quantitative relationships wereintroduced briefly for the three matter’s new analysis methods in this topic.Chapter2: A high performance liquid chromatographic method for thedetermination of residue methyl methacrylate （MMA） in PMMA denture basematerials was developed. And its dissolving characteristics were also analysedrespectively in the three extract solutions. Agilent TC-C₁₈column was used asStationary phase and acetonitrile-H2O mixture （70+30） was used as mobile phase inthe separation. MMA was determined at210nm. The linear relationship was obtainedbetween values of peak area and the concentration of MMA in the range of0.05μg/mL～100μg/mL with detection limit0.03μg/mL.Values of recovery wereranged from99.50%to102.00%. The method was sensitive and accurate. Thedissolving out amount of MMA was very large in the52°wine. It was lesser in thephysiological saline. And It was minimum （≤0.44mg/g） in the water.Chapter3: A new resonance light scattering（RLS） method for the determinationof bisphenol A was developed. In pH8.0Britton-Robinson buffer solution, the RLSvalue of safranine T had all been greatly improved at332nm and560nm because ofredox and ion-association reaction happen when mixing bisphenol A into thepotassium periodate and safranine T（ST） system. Since a good linear relationshipbetween resonance scattering enhancement value（ΔI） and bisphenol Aconcentration（ρ）: at λ=332nm and ρ=0.010～2.40μg/mL, ΔI＝356.93ρ（μg/mL）+19.77, r=0.998. And at λ=560nm, ρ=0.024～2.40μg/mL, ΔI＝245.45ρ（μg/mL）+1.46, r=0.999. Detection limit of2.86ng/mL and7.10ng/mL （3S/K） were obtainedrespectively. Recoveries were93.88%～103.33%and101.67%～105.00%by the 332nm wavelength, the relative standard deviation were3.52%～7.80%and4.23%～8.33%, respectively. The proposed methods had the advantage of simplicity, highsensitivity, low cost. And the methods had been applied to the determination ofbisphenol A in food packings and bottled drinking water with satisfactory results.Chapter4: The new method of inhibition resonance fluorescence was developedfor determination of H2S based on resonance fluorescence quenching of fluorescencemercury by hydrogen sulfide in the KH2PO4-NaOH buffer solution. Under theoptimized experimental conditions, a good linear relationship was obtained betweenthe ΔF of the system and concentration of H+2S in the range of7.38×10^-8～1.05×10^-6mol/L. It was ΔF=20.62c-5.35(c,10^-7mol/L), and the detection limit was2.21×10^-8mol/L. Sample recoveries were91.80%to97.45%. This method wassensitive, easy to operate, and the determination of hydrogen sulfide for biologicalmessenger with satisfactory results.