Antisense Tiny MiR-29b On The Growth Of Cancer Cells In Vitro Bladder

Objective: To observe microRNA-29b(miR-29b) antisense oligodeoxynucleotide(ASODN) on the impact of the growth of human bladder cancer cells BIU87andexplore antisense tiny miR-29b（AS-miR-29b）for the possible mechanism of thedevelopment of bladder cancer BIU87cells.Methods: Plasmid-mediated antisense oligodeoxynucleotide （AS-miR-29b） wasapplied to transfect into human bladder cancer the strains BIU87cells. After thetransfection was determined to successfully, applying Fluorescence Quantitative PCRto detect the transfected bladder cancer cells miRNA-29b of relative expression level.Then MTT colometric method, transwell and flow-cytometry method wasrespectively used to detect AS-miR-29b which impacted on BUI87cell’s proliferation,invasion and apoptosis. In addition, the AS-miR-29b transfection group was comparedwith the transfected nonsense sequence group and the blank control group.Results:1. Plasmid-mediated antisense oligodeoxynucleotide（AS-miR-29b）could besuccessfully expressed in bladder cancer BIU87cells, after the efficiency oftransfection detected by flow cytometry, the1:3group’s transfection efficiency wasthe highest, reaching55.7%.2. Real-time fluorescence quantitative PCR experiments showed that compared withthe negative control group and blank control group, the expression of transfectionAS-miR-29b was significantly upregulation, the difference was significant (P <0.05).3. After the MTT experiment, compared with the other two groups, it showed that theBIU87cells’ growth rate of the AS-miR-29b transfection group slowed down, cellproliferation was limited, there was a significant difference (P <0.05).4. After the Transwell experiment of invasion assay, the AS-miR-29b transfectiongroup showed that the cell count through the the Matrigel significantly reduced, thecapacity of invasion had diminished and the difference was significant (P <0.05). 5. Flow cytometric detection of apoptosis and necrosis, BIU87cell apoptosis andnecrosis proportion was significantly increased in AS-miR-29b transfection group,resulting in a significant difference (P <0.05).Conclusions:1. AS-miR-29b by transfection can effectively be transferred intoBIU87cells, and it’ s expression is upregulation.2. The over expression ofAS-miR-29b can play the role in the inhibition of cell proliferation, reduce cellinvasion force and accelerate apoptosis.3. The differential expression of miR-29bmay vitro bladder cancer cell growth function.