Multiphoton Microscopic Imaging Used In The Study Of Monitoring The Process Of Esophageal Squamous Cancer

Esophageal cancer is one of the most common cancer and leading cause of cancer death worldwide. Early detection is important to improve the quality of life in patient and reduce mortality. Based on two-photon excited fluorescence and second harmonic generation, multi-photon microscopy imaging technique can provide the considerable information on clinic diagnosis of early esophageal cancer. In this study, we first investigate images at different depths within mouse esophageal tissues, it provide some comprehensive information for the pathological diagnosis of tissues with stratified squamous epithelia. Then we also study the morphological change of human normal esophagus, carcinoma in situ, and early invasive carcinoma, the diagnostic features such as the appearance of cancerous cells, the absence of the basement membrane were extracted to distinguish between normal and cancerous esophagus tissue. At last, based on the pathological staging of esophageal cancer in China, we image the microstructure of intraepithelial carcinoma, intra-mucosal carcinoma and sub-mucosal carcinoma, our result show that the significant loss of stroma and the change of vessel in cancer tissue, the depth of tumor infiltration in different tissues. With the advancement of clinically miniaturized MPM and the multi-photon probe, combining MPM with standard endoscopy will therefore allow us to make a real-time in vivo diagnosis of early esophageal cancer at the cellular level.