Effect Of Mechanical Chest Compression CPR On Donor Heart

1. BackgroundWith the social development and economic progress, people’s living standards continue toimprove, followed by an increase in a variety of obesity-related diseases. Heart transplant is the terminaltreatment of high blood pressure, coronary heart disease and structural heart disease. Donor heart has thelimitation on the quantity and quality for heart transplantation. Currently, many studies devote to findingan approach about how to get enough hearts and improve cardiac function of heart. This is a prerequisitefor a heart transplant success. Although the Number of heart transplant center in the world is constantlyincreasing, the number of heart transplantation has not obviously increased, which is the consequence ofbottleneck of lack of donor hearts and protection issues. It is a key step to make successful hearttransplantation by maximally maintaining the heart function. According to cardiac anatomic structure andphysiological characteristics, the heart myocardial protection measures should be aimed at the in vitropreservation methods. In addition, it will provide the best protective function of cardiac structure andmuscle via saving and reducing energy consumption in warm and cold ischemia Thus, the way of donorheart preservation has an important clinical significance.2. Materials and MethodsFifteen Swedish pigs were randomly divided into two groups (15pigs/group): artificial cardiacmassage group (CRP) and mechanical cardiac massage group (MCPR). After establishment of artificialcardiac arrest, the hearts from these pigs was handles by CPR or MCPR following by LUCAS. To explorewhich kind of methods will provide the less effect of the donor heart, we determine to detect the blood flow of donor heart pulmonary artery,coronary artery endothelial function.3. ResultsThe results showed that the application of the LUCAS saving donor cardiac function wassignificantly better than conventional preservation donor heart, endothelial cells function to maximizeprotection. and cardiomyocyte apoptosis decreased in LUCAS preservation group. Meanwhile, superoxidelevels within cardiomyocyte in the LUCAS treatment was significantly lower than conventionalpreservation group. Recent studies showed that the non-receptor tyrosine kinase c-Src, is not only involvedin cell proliferation, differentiation, apoptosis and other important physiological functions, but alsoinvolved in the regulation of intracellular oxidation-reduction state. Western blot results showed that theactivity of c-Src significantly increased upon LUCAS treatment group compare to the control.4. ConclusionThese results suggest that treatment by LUCAS donor heart, coronary endothelial function,preservation of cardiac function and myocardial cells c-Src activity has been at a high level, which affecton the level of myocardial cellular redox regulation and the intracellular super-the level of oxide, inhibitionof apoptosis of myocardial cells. Thus, an automatic cardiac compressions could provide better donorconditions for heart transplantation that contribute to providing more donor sources.