Studies On The Chemical Constituents Of Stephania Viridiflavens And Bergenia Purpurascens

The master’s thesis consists of three chapters. First of all, in chapter onediscussed the study of chemical constituents from Stephania viridiflavens; and then,the chapter two discussed the study of chemical constituents from Bergeniapururascens herb; lastly, the chapter three is based on literatures to summarize theresearch progress of the chemical constituents、bioactivities、content determination、and clinical application of Bergenia pururascens comprehensively.Chapter1Chemical constituents of Stephania viridiflavensStephania viridiflavens (蛇莲), one folks Chinese medical herb, is one of theMenispermacaeae Stephania plants, possess of the pharmacological activities ofdetoxication、analgesia and so on, By use of various chromatography technologycombine the purification technology,36compounds were isolated from the tubers and31of them were identified via spectrum analysis. For respectively：Tetrahydropalmatine(1)、Corydalmine (2)、Xylopinina (3)、Coritenchina (4)、Corypalmine (5)、Crebanine (6)、Isocorydine (7)、 Phanostenine (8)、Corydine (9)、Dicentrine (10)、Stephanine (11)、Menisperine (12)、Dehydrocrebanine (13)、Corytuberine (14)、 Sinoacutine (15)、 Sebiferine (16)、 Nortenuipine (17)、Cepharanthine (18)、Sinomenine (19)、Sukhodianine (20)、Jatrorrhizine (21)、Columbamine (22)、Palmatine (23)、Isocorydione (24)、Cassythicine (25)、Dehydrodicentrine (26)、13a(S)-7(S)-N-methyl-tetrahydropalmatine (27)、Terephthalates(28) Stepharine (29)、Coreximine (30)13a(S)-7(R)-N-methylxylopinine(31).Among them, the compounds27and31are new native compound, the co mpounds2、4-6、8、10-22、24-31were isolated from this species for the firsttime.4,12,16,17,28,30,31was isolated from this genus for the first time.Structure types mainly include aporphine、proaporphine、berberine、bisbenzylisoquinoline and morphine alkaloids originally.Chapter2The chemical constituents of Bergenia pururascensBergenia pururascens (岩白菜), is one Chinese medical herb possess ofantibechic and expectorant, also the resource plant of yield Bergernine. In order torealize the chemical constituents of Bergenia pururascens deeply, and search for thecompounds which are similar to the structure of bergenin. By use of variouschromatography technology,9compounds were isolated from the methanol extractionvia column chromatography and purification: Bergenin (1)、Oleanic acid (2)、Arbutin(3)、Afzelechin (4)、11-O-galloyl bergenin (5)、Gallic acid (6)、Breynioside A(7)、Diethyl disulfoxide (8)、(+)-Catechin (9)、Phenols and flavonoid included in thesecompound types. Among them, compounds4、7and8were isolated from this plantfor the first time, and compound8was the sulfocompound isolated from Bergeniaplants for the first time.Chapter3The research progress of Bergenia plantsIn this part, we carried out a comprehensive summary that aim to the chemicalconstituents and bioactivities，consist of structure reformation and their derivates’bioactivities of Bergenia plant on the basic of literatures. Meanwhile, we also trimmedthe literatures about botany resource、clinical application and the relevance.