Evaluation Of Adjuvants Effects Of Water Soluble Polysaccharide From The Stem Of Physalis Alkekengi L.var.francheti (Mast) Makino In A Recombinant Protein Vaccine

Physalis alkekengi L. var. francheti (P. alkekengi)(Solanaceae) is an edible plant widelyused in the oriental country and known for its medicinal and therapeutic attributes. The fruitof P. alkekengi is mostly the focus of many individuals as well as researchers. This researchwork however concentrated on how the stems of can be utilized efficiently.The main aim ofthis research was to evaluate the efficacy of the water soluble polysaccharide (WSP)extracted and purified from stems of P. alkekengi used as an adjuvant, in mice.Vaccine (rP-HSP90C) used was a recombinant protein containing epitope C (LKVIRK) fromHSP90of Candida albicans (C. albicans). Male ICR mice were immunized subcutaneouslywith rP-HSP90C alone, rP-HSP90C/WSP (100μg,200μg）, and saline two times at14-dayinterval. The mice were sacrificed two weeks after the last immunization. The ELISAtechnique was employed to evaluate the changes in titre values from the serum drawn fromeach mouse based on the different concentrations of WSP to the presence of IgG, IgG1, andIgG2respectively. It was observed that WSP significantly enhanced the antibody titers (IgG,IgG1andIgG2b) in serum (P<0.05).WSP was also seen to promote the release of IL-10, IL-12, thus inducing Th1and Th2immune response (P<0.05).WSP may be a favorablecandidate for vaccine enhancement in the near future.