The Expression Of Germ Cell Nuclear Factor And OCT-4in Cervical Cancer

Objective:To study the expression of GCNF and OCT-4gene/protein in normal cervical tissues,cervicalintraepithelial neoplasia (CIN) tissues and cervical squamous cell cancer tissues and the relationships of theexpression between GCNF and Oct-4, in order to provide a new target and reference for clinical diagnosisand treatment of cervical cancer.Methods:(1)Collecting20cases of normal cervical tissues,70cases of cervical intraepithelial neoplasia(CIN) tissues, and29cases of cervical squamous cell cancer tissues paraffin blocks in the PathologyDepartment of the First Auxiliary Hospital of the Shihezi University, to detect the expression of GCNF andOCT-4protein by immunohistochemical SP method;(2)Detecting the level of expression of OCT-4andGCNF mRNA of9cases of normal cervical tissues,10cases of CINⅠ,9cases of CIN Ⅱ,10cases of CINⅢand9cases of cervical squamous cell cancer tissues by RT-PCR method.The database was set up in Excel,and statistically processed by SPSS13.0software.Results:(1)The expression of GCNF protein in the positive control adult testis was strongly positive andmainly located in the nuclei of round spermatids or pachytene spermatocytes, with brown staining; innormal cervical tissues it was moderately positive, with brownish yellow, located in the nucleus and theexpression rate was90.0%(18/20); in cervical intraepithelial neoplasm(CIN)tissues it was moderatelypositive or weakly positive, the expression rates in CIN Ⅰ, CIN Ⅱa nd CINⅢ were78.3%(18/23),45.0%(9/20) and33.3%(9/27) respectively; the majority of cervical squamous cell carcinoma tissues werenegative, a few was weakly positive and the expression rate was13.8%(4/29).(2)The result of GCNF RT-PCR gel electrophoresis showed that GCNF band was200bp and observed innormal cervical tissues,cervical intraepithelial neoplasia (CIN) tissues and cervical squamous cell cancertissues. But its brightness from normal cervical tissues to cervical squamous cell cancer tissues graduallyweaked. The GCNF/GAPDH gray ratio in normal cervical tissues,CINⅠ, CINⅡ, CIN Ⅲand cervicalsquamous cell carcinoma tissues were0.827±0.013,0.687±0.016,0.621±0.038,0.606±0.023and0.464±0.036respectively, each tissue was repeated for three times and took the average gray ratio as the finalgray ratio. The difference among normal cervical tissues,CINⅠ, CIN Ⅱ, CIN Ⅲand cervical squamous cellcarcinoma tissues has statistical significance.While the difference between CINⅡ and CINⅢ has nostatistical significance.(3)The expression of OCT-4protein in the positive control seminoma was strongly positive and mainlylocalized in the nucleus, with brown particles. But it was negative in normal cervical tissues, cervicalintraepithelial neoplasia (CIN) tissues, as well as the cervical squamous cell cancer tissues.(4)OCT-4RT-PCR gel electrophoresis result showed that OCT-4band was218bp and observed in positivecontrol seminoma.While it was not observed in CIN Ⅰ, CIN Ⅱ, CIN Ⅲand cervical squamous cell cancertissues.Conclusion:(1)that GCNF gene mainly expressed in the basal and spinous layer cell of normal cervicaltissue suggested that GCNF gene may be associated with the normal epithelial cell differentiation.(2)That GCNF gene did not express in cervical squamous carcinoma tissues suggested that it GCNF gene may beassociated with abnormal differentiation of the cancer cells.(3)The expression between GCNF and OCT-4in cervical squamous cell carcinoma tissues is no relationship.