| Objective：To investigate the intervention effects of ginkgo bilobaextract (GBE) pretreatment and the acute stage administration onepileptic rats and its relationship with the expression ofN-methyl-D-aspartate receptor2A (NR2A) and neuropeptide Y (NPY), inorder to probe the anti-epileptic mechanism of GBE.Methods：An animal model of complex partial epilepsy wasestablished in male SD rats by intracerebroventricular injection of Kainicacid(KA).90SD rats were randomly divided into sham operation group(group A), GBE control group (group B), epilepsy model group (group C),GBE pretreatment group (group D), and GBE acute administration groups(E). Each group was further divided into three subgroups (6hours,12hand24h after seisures),6rats of each subgroup. Morphologic changes ofthe hippocampal neurons were observed by HE staining, while the NR2Aexpressions of hippocampal CA1and NPY expressions of thehippocampal dentate gyrus were measured by immunohistochemical techniques.Results：Seizure levels and neuronal damage of group D and group Ewere lower than those of group C. In hippocampal tisse, NR2A and NPYexpression of three subgroups in Group C increased significantly thanthose in group A(P<0.05); NR2A expressions of three subgroups inGroup D and group E were lower than those in group C(P<0.05), andNPY expressions of three subgroups in Group D and group E increasedsignificantly than those in group C(P<0.05). NR2A protein expressionswere decreased and NPY protein expressions were increased in group Dcompared with those in group E(P<0.05). No statistically difference ofNR2A and NPY expression were found between group B and group C(P>0.05). Downturns of NR2A expression and upturns of NPYexpression were showed from6h to24h time point after seisures in thesame groups.Conclusion：GBE have some antiepileptic effects, and the effectsof pretreatment are better than that of acute stage administration. Theanti-epileptic effects of GBE are concerned with lessen of neuronicdamage, the mitigation of seizure degree, the down-regulation of NR2Aexpression and the up-regulation of NPY expression. |
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