Experimental Study Of PDGF-α Receptor ASODN On Preventing Proliferative Vitreoretinopathy

Objective: To examine the effect of PDGF-α receptor on proliferativevitreoretinopathy(PVR)in rabbits.Methods: Different concentrations of PDGFR-α ASODN/lipofectamineTM2000was mixed to make PDGFR-α ASODN/lipofectamineTM2000complex,with the final ratios of1:1、1:2.5and1:5respectively. All the complex wereincubated with culture human retinal pigment epithelium for24hours beforetransfection. After establishment of proliferative vitreoretinopathy on the righteyes of24pigmented rabbits, the rabbits were received intravitreous injectionsand were divided into group A (RPE cells)、 group B and C(1.0、2.0μmol/LPDGFR-αASODN lipofectin transfection of RPE cells) with8eyeseach.The fellow eyes were injected with a volumetric equivalent dose of BSS（0.1ml）as control. The degree of proliferative vitreoretinopathy were estimatedby indirect ophthalmoscope examination on postoperative1、2、3、4week; At theconclusion of an experiment, the fundus changes of the selected eyes wereestimated by histopathology; and the density of PDGF-α was detected byimmunohistochemistry.Results: When PDGFR-α ASODN/lipofectamineTM2000ratio was1:2.5, the highest transduction efficiency appeared.The retina was detached after1~2weeks, the PVR had progressed to higher stages with time in group A. In theeyes injected B and C, the PVR also developed; however, the severity of PVRwas lower than that of group A (all P<0.05), group C was more lower thangroup B. The histologic examination showed the red-dyed streak, reticulatecollagen fibers, destroy of retinal structure in the4th week of mode1A, theseverity of group B and C was lower than that of group A, and group C wasmore lower than group B. The control group had no significant funduschanges.The retinal layer cells and proliferated of fibers PDGF-α was highstrength brown coloring (++++) in group A, that was brownish yellow coloringof moderate-intensity (+++)in group B, was weaker brown coloring (++)ingroup C; The Surface of retinal ganglion cells of the normal control group andretinal pigment epithelium cells was visible weak brown coloring (+).Conclusions: PDGF-α receptor antisense oligonucleotides can inhibit thedevelopment of experimental proliferative vitreoretinopathy, which may berelated to the suppression of PDGF-α in retinal cells.