Objective:The islet microvascular endothelial cells are next to β cells and they effects thefunctions of β cells.To investigate effects of silencing MAS receptor by small interferingRNA(si-RNA) on the cell proliferation, cell cycle and dysfunction of MS-1cellsMethods:Three siRNAs targeting Mice MAS receptor were synthesized, which weretransfected into MS-1cells using a liposome approach. The expressions of MAS receptor,eNOS, ICAM, VCAM mRNA in MS-1cells after transfection with MAS si-RNA weredetermined by real-time fluorescence quantitative PCR(RFQ-PCR).The expression ofeNOS protein is detected by western blotting. The cell growth was tested by cck-8.Theproliferation and cell cycle and transfection efficiency were detected by flowcytometry(FCM), respectively.Results:As compared with the blank control (untransfectedwith siRNA), the expression levels of eNOS mRNA and MAS mRNA were decreased8.7%and15.8%after transfection with MAS siRNA, respectively. The level of proteinexpression of MAS declined. The expression levels of ICAM-1, VCAM-1mRNAincreased,showing no statistical significance with the blank control. The cell proliferationwas promoted (P<0.05), the cell cycle was arrested at S phase (P<0.05), and theproliferation index was increased.(P<0.05).【Conclusion】MAS siRNA can effectivelysuppress the expressions of MAS mRNA and eNOS mRNA in MS-1cells, and unusuallypromote the cell proliferation in vitro. |