The Research On The Antitumor Activity Of PTD4-Apoptin Fusion Protein On Human Non-small Cell Lung Cancer

Objective: To observe the anti-tumor activity of PTD4-Apoptin fusion protein onHuman non small cell lung cancer in vitro and in vivo.Methods: A549、NCI-H1299and HUVEC were cultured in PTD4-Apoptin fusionprotein in vitro. Cell viability was analyzed by MTT assay, AnnexinV-FITC/PIdouble-staining assay was used to test the apoptotic effects. We construct the nude micemodels of Human non small cell lung cancer by A549cells.The nude mice bearing A549were treated with the PTD4-Apoptin fusion protein and the inhibitory effect on the tumorgrowth was observed. PTD4-GFP protein and PBS was used as control.Results: The MTT studies showed PTD4-Apoptin fusion protein could inhibit theproliferation of A549、 NCI-H1299cells in a dose-dependent manner in vitro.AnnexinV-FITC/PI double-staining assay showed PTD4-Apoptin fusion protein couldinduce apoptosis in A549、NCI-H1299cells but not in HUVEC. The tumor growth treatedby PTD4-Apoptin fusion protein was slower than the control groups. Animal experimentsshowed the PTD4-Apoptin fusion protein could penetrate into the tumor of the mice via theepidermal tissue and inhibited the growth of the tumor.Conclusions: PTD4-Apoptin fusion protein can inhibit the growth of A549andNCI-H1299cells in vitro; PTD4-Apoptin fusion protein can inhibit the tumor growth ofnude Mice bearing A549. Objective: To observe the anti-tumor Effect of RNA interference targeting ADAM10in Mice Bearing U14Cervical cancer.Methods: Cervical cancer U14tumor model was established and the KM mice bearingU14cells were divided into3groups at random after the tumor grew up to5mm in diamete.To observe the inhibitory effect on tumor growth, the pGenesil1-ADAM10shRNAexpression vector was injected into the KM mice, pGenesil4-HK shRNA and saline wereused as control. The mice were killed by hauling necks after treatment and the expression ofADAM10on the tumor was assessed by immunity histochemistry technology.Results: The tumor growth injected by pGenesil1-ADAM10shRNA was slower thanthe control groups. The immunocytochemistry results showed that the expression ofADAM10protein was inhibited significantly by injecting pGenesil1-ADAM10shRNAplasmid, and the injection of pGenesil4-HK shRNA and saline could not reduce theexpression of ADAM10protein.Conclusions: pGenesil1-ADAM10shRNA plasmid can inhibit tumor cellsproliferation in U14tumor-bearing mice by blocking the expression of ADAM10.