Effect Of Estrogen On CXCL10Expressed By Hepatic Stellate Cells LX-2and Migration Of LX-2Induced By CXCL10

Objective：To determine the effect of estrogen on the expression of chemokine CXCL10inhepatic stellate cell line LX-2, as well as the alteration of migration of LX-2induced bychemokine CXCL10, and to explore the protective mechanism of estrogen in theprogression of hepatic fibrosis disease.Methods：Hepatic stellate cell line LX-2was treated with estradiol for different time and then theeffect of estrogen on the expression of chemokine CXCL10was detected by real-time PCRand western blot. The change of migration of LX-2induced by chemokine CXCL10wasexamined by transwell chemotactic test.Results：1. When hepatic stellate cell LX-2was treated with estradiol for3h and12h, the relativeCXCL10mRNA expression(0.33±0.02and0.59±0.04, respectively) were bothsignificantly lower than the control group (1.00±0.02), and the differences werestatistically significant (both p<0.01). The relative CXCL10mRNAexpression level at24h(0.99±0.00) was similar to the control group (p>0.05). When comparing the12h groupwith the3h group, the24h group with the12h group, the relative CXCL10mRNAexpression levels were both elevated (both p<0.05).2．After treatment with estradiol for3h and12h, the relative CXCL10proteinexpression of LX-2(0.64±0.03and0.84±0.12, respectively) were downregulated(compared with the control group,1.00±0.00), and the differences were statisticallysignificant (p<0.01and p<0.05, respectively). The relative CXCL10protein expressionlevel at24h (0.97±0.09) was similar to the control group and the difference was notstatistically significant (p>0.05). The relative CXCL10protein expression level wasupregulated when comparing the12h group with the3h group (p<0.05). However, therelative CXCL10protein expression level didn’t change when comparing the24h group with the12h group (p>0.05).3. After adding10ng/ml CXCL10to the lower chamber, migration LX-2cells wassignificantly increased compared with the control group (227.67±8.41vs110.11±7.88).The difference was statistically significant (p<0.01). When we added10-8mol/l estradiol tothe upper chamber to inhibit the migration of LX-2, migration cells induced by10ng/mlCXCL10was decreased (154.19±6.11), and the difference was statistically significantcomparing with10ng/ml CXCL10group (p <0.01).Conclusion：Estrogen can inhibit the expression of CXCL10in hepatic stellate cell line LX-2，which in turn inhibit LX-2migration induced by CXCL10. The potential protective effectsof estrogen in the process of liver fibrosis may be partly achieved by inhibiting theexpression of the chemokine CXCL10and LX-2migration induced by CXCL10, thusdelaying the progression of hepatic fibrosis.