In Vitro Culture Of Human Musculus Constrictor Pharyngis Superior Cells With Modified Polyurethane Scaffold For Tissue Engineering Research

A good compatibility polymeric scaffolds have been widely employed to create a livingconstruct that would mimic the complexities of human hypopharynx tissue function in the field oftissue engineering.Isolation of human pharyngeal constrictors cells from body tissue of nine Volunteershypopharyngeal carcinoma patients by explanting method. Collect4th generation cells forgrowthing on modified polyurethane (PEU) scaffolds with linearly aligned microgrooves.Identification of the cell morphology and observation the status of the proliferation anddifferentiation were carried out by morphology observation and immunohistochemistry analysisTo construct such a tissue, a polyurethane (PEU) scaffold with linearly aligned microgrooveswas fabricated that could direct myoblast adaptation under stringent conditions. The closely spacedmicrogrooves fabricated by a modified replica molding process guided linear cellular alignment.Considered the low hydrophilic property, PEU scaffold is grafted with silk (PEU-SF) in order toimprove its cell compatibility. It is believed to promote the skeletal regeneration if silk is graftedonto artificial substitutes for hypopharynx tissue engineering.Examination of the myoblasts byimmunofluorescence microscopy demonstrated that the microgrooves with appropriate height-to-width ratios were required for practically complete linear alignment of myoblasts. Electronmicroscopy (SEM) showed that the scaffold to be able to support the growth of hypopharynxskeletal cells. The findings demonstrate that cells seeded on such scaffolds hold promise forproviding an efficient means for guided hypopharynx muscle tissue construction.The next step isanimal experisement, which hold promise for guiding human explants with such scaffold.Cells were cultured in Dulbecco’s modified Eagle’s medium,(DMEM), supplemented with20%foetal bovine serum (FBS) under conditions of37°C,5%CO2by explanting method as thebest conditions for vitro culture.Silk fibroin (SF) grafting greatly improved scaffold’s biological activitywhich led to the high cell biocompatibility. This paper reviews the progresses of human hypopharynxskeletal muscle cells in ex vivo isolation, proliferation, identification and the feasibility to be usedin tissue engineering research.